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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | GT1-7 is a conditionally immortalized mouse hypothalamic neuronal cell line established by R.I. Weiner and colleagues in 1990. Given that native gonadotropin-releasing hormone (GnRH) neurons are sparsely distributed and extremely rare, this cell line was generated by inducing targeted tumorigenesis in a transgenic mouse population. Specifically, researchers introduced a hybrid transgene construct—comprising the rat Gnrh1 promoter and the coding sequence for the Simian Virus 40 (SV40) large T-antigen oncogene—into (C57BL/6J × BALB/cJ) F2 hybrid mouse embryos. Subsequently, tumors arising in the anterior hypothalamus were harvested, dissociated, and subjected to clonal selection, ultimately yielding the stable and highly differentiated GT1-7 subclone. GT1-7 has become the preferred in vitro model for neuroendocrinology research worldwide. These cells retain characteristic neuronal processes, synthesize high levels of endogenous Gnrh1 mRNA, and exhibit pulsatile GnRH secretion in response to physical depolarization stimuli, thereby mimicking authentic physiological neurosecretory processes. |
| Tissue | Brain |
| Cell Type | Mature differentiated GnRH Neuron |
| Disease | None (Derived via targeted transgenic neurotumorigeneis of normal cells) |
| Morphology | Neuronal-like; adherent polygonal cells that actively extend complex neurites |
| Gender | Unspecified / Mixed parental background |
| Age | Embryonic / Postnatal Transgenic Development |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 or 2 (Repositories evaluate integrated SV40 oncogene sequences under standard biohazard monitoring rules) |
| Applications | 1. Serve as a "gold-standard" in vitro model for simulating the physiological properties, pulsatile activity, and regulatory networks of hypothalamic GnRH neurons. 2. Elucidate neuroendocrine signaling pathways driven by upstream neuropeptides. 3. Investigate the transcriptional mechanisms regulating reproductive development and mammalian reproductive cascades. 4. Screen for potential neuroprotective small molecules, hormonal modulators, and environmental endocrine disruptors. 5. Map depolarization-induced calcium (Ca2+) flux and vesicle exocytosis dynamics in brain cells. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C (Liquid nitrogen vapor phase only) |
| Characteristics | |
| Tumorigenic | Yes, derived originally from an induced anterior hypothalamic tumor mass. |
| Transformant | SV40 large T antigen under the transcriptional control of a rat Gnrh1 promoter snippet. |
| Secretory Profile | Synthesizes and continuously releases mature Gonadotropin-Releasing Hormone (GnRH) in a highly rhythmic, episodic fashion. |
| Receptor Profile | Endogenously expresses key neuroendocrine targets (including the orexin-1 receptor, OX1R) but completely lacks expression of orexin-2 receptor (OX2R) transcripts; simultaneously tests positive for estrogen receptors α and β (ERα/ERβ). |
| Mycoplasma Test | Negative |
| Viral Clearance | Tested negative via comprehensive CLEAR panel PCR panels for common murine infectious agents. |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the spent complete culture medium from the culture flask. 2. Gently rinse the cell monolayer with sterile, Ca2+/Mg2+-free PBS to neutralize residual serum containing active trypsin inhibitors. 3. Add 2.0 to 3.0 mL of a 0.05% to 0.25% trypsin-0.53 mM EDTA solution, ensuring the cell layer is completely submerged. 4. Incubate at room temperature or 37°C for 5 to 15 minutes; monitor under an inverted microscope until the cells become rounded and detach. Note: Avoid physical disturbances such as tapping or shaking the flask during incubation to minimize cell clumping. 5. Immediately add 6.0 to 8.0 mL of pre-warmed complete culture medium to the flask to stop trypsin activity, and gently pipette up and down to create a uniform cell suspension. 6. Centrifuge at approximately 125 × g for 5 to 7 minutes, discard the supernatant, resuspend the cell pellet in fresh medium, and distribute into new vessels. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio spanning 1:4 to 1:7 is standardly recommended for routine maintenance. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 95% Fetal Bovine Serum (FBS) + 5% DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02616 | Cas9 Stable Cell Line - GT1-7 | Inquiry |
| CSC-RR00923 | GFP Reporter Cell Line - GT1-7 | Inquiry |
| CSC-RR00960 | Luciferase Reporter Cell Line - GT1-7 | Inquiry |
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