DC2.4 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	DC2.4 is an immortalized murine dendritic cell line established by Z. Shen, G. Reznikoff, and their colleagues in 1997. This cell line was generated by transducing primary bone marrow cells from C57BL/6 mice with a retroviral vector that drives the co-expression of murine granulocyte-macrophage colony-stimulating factor (GM-CSF) and the v-myc and v-raf oncogenes. Exhibiting typical dendritic cell morphology, DC2.4 cells serve as a vital in vitro model in immunological research. They are widely used to study antigen presentation, cross-priming pathways, and dendritic cell maturation cascades, as well as to screen potential vaccine candidates or cancer immunotherapies.
Tissue	Bone Marrow
Cell Type	Dendritic Cell (Antigen-Presenting Cell / APC)
Strain	C57BL/6
Disease	Normal / Non-malignant tissue origin (Immortalized engineered line)
Morphology	Dendritic / Stellate with dendritic-like processes; grows as an adherent monolayer with occasional loosely attached clustering.
Gender / Age	Unspecified
Growth Mode	Adherent
Applications	1. A gold-standard model for analyzing the mechanisms of MHC class I and II antigen processing and presentation. 2. Elucidating the maturation process of dendritic cells using exogenous stimuli such as interferon-gamma (IFN-γ). 3. Modeling T-cell activation, cross-priming, and downstream antigen-specific CD8+ cytotoxic T-lymphocyte responses. 4. Preclinical vaccine development, evaluation of targeted delivery platforms, and validation of nucleic acid-mediated cancer immunotherapies. 5. Investigating host-pathogen interactions, innate immune pathways, and exosomal RNA communication networks.
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase only)
Characteristics
Tumorigenic	No, retains base non-malignant physiological immune properties, although it carries stable oncogenic driving fragments.
Transformant	Murine GM-CSF paired with v-myc and v-raf oncogenes integrated via retroviral transfection.
Phenotypic Baseline	It exhibits an immature dendritic cell phenotype under standard expansion conditions. It constitutively expresses the cell surface markers CD11c and MHC class I molecules, but shows low baseline expression levels of MHC class II molecules and costimulatory molecules such as CD40 and CD80.
Maturation Potential	Highly responsive to activation. Upon stimulation (e.g., with IFN-γ or LPS), MHC class II molecules, CD40, CD80, and CCR7 are significantly upregulated, accompanied by the high-level secretion of pro-inflammatory cytokines, including IL-6, IL-12, and TNF-α.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Aspirate and completely discard the spent complete culture medium from the culture flask. 2. Gently rinse the cell monolayer with sterile, Ca2+/Mg2+-free PBS to remove residual traces of serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.53 mM EDTA solution, ensuring the cell layer is completely covered. 4. Incubate at 37°C for 2 to 5 minutes; monitor under an inverted microscope until the cells become rounded, loosen, and completely detach from the plastic surface. 5. Quickly add an equal volume of pre-warmed complete culture medium to neutralize trypsin activity. 6. Centrifuge the cell suspension at approximately 200 × g for 5 minutes, discard the supernatant, gently resuspend the cell pellet in fresh complete culture medium, and distribute the suspension into new culture vessels.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	Passage cells that are in an optimal growth state and have reached confluence at a standard ratio of 1:3 to 1:6. Do not allow the cells to become over-confluent, as overcrowding may induce spontaneous differentiation.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete culture growth medium + 10% DMSO (or standard commercial Freeze Medium CM-1)

Cat.No.	Product Name	Price
CSC-RO02569	Cas9 Stable Cell Line - DC2.4	Inquiry
CSC-RR00895	GFP Reporter Cell Line - DC2.4	Inquiry
CSC-RR00944	Luciferase Reporter Cell Line - DC2.4	Inquiry

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* For research use only. Not intended for any clinical use.

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