Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | LU65 is a human giant-cell carcinoma cell line derived from a primary lung tumor specimen obtained from a Japanese male patient. LU65 cells exhibit an epithelial morphology and grow as an adherent monolayer under standard culture conditions. A key biological feature of this cell line is its high expression and secretion of granulocyte colony-stimulating factor (G-CSF), making it a classic model for studying G-CSF-producing human tumors and tumor-associated leukemoid reactions. Cytogenetically, the cell line displays an aneuploid karyotype characterized by complex chromosomal alterations. At the molecular level, LU65 harbors somatic mutations in driver genes such as KRAS and TP53. Due to its unique cytokine secretion profile and robust growth capacity, LU65 serves as a valuable in vitro model for investigating the pathogenesis of giant-cell carcinoma of the lung, studying G-CSF-driven signaling within the tumor microenvironment, evaluating candidate chemotherapeutic agents, and conducting functional genomic research. |
| Tissue | Lung |
| Disease | Lung Giant Cell Carcinoma; Non-Small Cell Lung Cancer (NSCLC) |
| Morphology | Epithelial |
| Gender | Male |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the biology of pulmonary giant cell carcinoma and the mechanisms underlying G-CSF (granulocyte colony-stimulating factor) production by such tumors; 2. Study of tumor-induced leukemoid reactions and paraneoplastic cytokine signaling; 3. Evaluation and screening of novel chemotherapeutic agents and targeted therapies; 4. Functional genomic analysis, target identification, and screening/validation using CRISPR/Cas9 technology. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid |
| Tumorigenic | Yes, forms tumors in immunocompromised (nude) mice |
| Genetic Profile | KRAS mutation; TP53 mutation |
| Expression Markers | Secretes Granulocyte Colony-Stimulating Factor (G-CSF); cytokeratin positive |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (without Ca2+/Mg2+) to completely remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.03% EDTA solution and incubate at 37°C until the cell monolayer detaches (typically 2 to 5 minutes). 4. Add fresh complete growth medium to neutralize the trypsin and gently pipette up and down to prepare a single-cell suspension. 5. Subculture the cell suspension into new culture vessels. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0345 | GFP Reporter Cell Line - LU65 | Inquiry |
| CSC-RO01509 | OVAL Stable Cell Line - LU65 | Inquiry |
| CSC-RO01762 | GFP/OVAL Stable Cell Line - LU65 | Inquiry |
| CSC-RO02015 | Luc/OVAL Stable Cell Line - LU65 | Inquiry |
| CSC-RO02268 | GFP/Luc/OVAL Stable Cell Line - LU65 | Inquiry |
The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.
Copyright © 2026 Creative Biogene. All rights reserved.