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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | Panc02 is a mouse pancreatic cancer cell line established in 1984. It was initially induced in male C57BL/6 mice via 3-methylcholanthrene (3-MCA) chemical carcinogenesis. Panc02 cells exhibit a typical epithelial-like morphology and grow as an adherent monolayer. Highly malignant and intrinsically resistant to many standard chemotherapeutic agents, Panc02 is the preferred homology model for pancreatic ductal adenocarcinoma (PDAC) research worldwide. Due to its complete homology with the immunocompetent C57BL/6 mouse, it serves as an important in vivo and in vitro platform for studying pancreatic tumor immunology, evaluating immune checkpoint blockade therapies, and testing novel immunotherapeutic interventions within the intact host immune microenvironment. |
| Tissue | Pancreas |
| Disease | Mouse Pancreatic Ductal Adenocarcinoma (PDAC) |
| Morphology | Epithelial-like |
| Gender | Male |
| Age | Unspecified / Adult |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Establish a homologous animal model of pancreatic ductal adenocarcinoma in immunocompetent C57BL/6 mice. 2. Elucidate the dynamic changes in the pancreatic tumor immune microenvironment and myeloid-derived suppressor cells (MDSCs). 3. Evaluate targeted therapy, gemcitabine resistance mechanisms, and combination therapy regimens. 4. Preliminary screening of cancer vaccines, viral vectors, and immune checkpoint blockade therapies. 5. Investigate metastatic signaling pathways using orthotopic or subcutaneous transplantation models. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C (Liquid nitrogen vapor phase) |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic and consistently develops metastatic tumors when engrafted into syngeneic C57BL/6 mice |
| Induction Agent | Chemically transformed using 3-methylcholanthrene (3-MCA) |
| Phenotype | Exhibits robust baseline resistance to multiple chemotherapeutic modalities; retains high invasive capability |
| Growth Kinetics | Rapidly proliferative; under optimal conditions, the standard doubling time is 22 to 28 hours. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture supernatant. 2. Gently wash the cell monolayer with calcium- and magnesium-free Dulbecco's phosphate-buffered saline (DPBS) to remove any traces of serum containing trypsin inhibitors. 3. Add 2.0 to 3.0 mL of 0.25% trypsin-0.53 mM EDTA solution (or an equivalent dissociation agent, such as Accutase) to cover the cell monolayer. 4. Incubate at 37°C for 3 to 8 minutes; observe under an inverted microscope until the cell layer is completely detached and dispersed. 5. Add an equal volume of complete culture medium to completely neutralize the trypsin. 6. Centrifuge the suspension at approximately 300 × g for 3 minutes, aspirate the liquid, gently resuspend the cell pellet in fresh culture medium, and evenly distribute it into new flasks. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | A split ratio of 1:4 to 1:8 is recommended for routine maintenance. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete growth medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO) |
| Cat.No. | Product Name | Price |
|---|---|---|
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| CSC-RR00871 | GFP/Luc Reporter Cell Line - PANC02 | Inquiry |
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| CSC-RO02141 | Luc/OVAL Stable Cell Line - Panc02 | Inquiry |
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