BT-20 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	BT-20 is a human breast adenocarcinoma cell line established by E.Y. Lasfargues and L. Ozzello in 1958, derived from a primary breast tumor in a 74-year-old Caucasian female. It is one of the earliest and most widely used models for triple-negative breast cancer (TNBC). The cell line is negative for the estrogen receptor (ER), progesterone receptor (PR), and HER2, though it expresses an ER mRNA variant lacking exon 5. These cells grow as an adherent epithelial monolayer with a doubling time of approximately 66 to 70 hours and can form grade II adenocarcinomas in nude mice. BT-20 harbors a homozygous TP53 p.Lys132Gln mutation, two heterozygous PIK3CA mutations, and a homozygous CDKN2A deletion; furthermore, the cell line exhibits microsatellite stability. It is extensively utilized in research concerning TNBC biology, apoptosis and TRAIL sensitivity, EGFR and PI3K signaling pathways, and three-dimensional spheroid culture.
Tissue	Breast / mammary gland (primary tumor, in situ)
Disease	Breast Adenocarcinoma; infiltrating ductal carcinoma of no special type; triple-negative (ER-, PR-, HER2-)
Morphology	Epithelial
Gender	Female
Age	74 years
Ethnicity	Caucasian
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Triple-negative breast cancer biology and molecular subtyping 2. Apoptosis induction and TRAIL / death receptor sensitivity studies 3. EGFR and PI3K pathway alteration analysis in breast cancer 4. Multidrug resistance and chemosensitization research 5. Three-dimensional spheroid and immuno-oncology co-culture models 6. Development of subcutaneous xenograft models in immunodeficient mice
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes, forms grade II adenocarcinomas in nude mice following subcutaneous inoculation
Karyotype	Chromosomes N3, N4, N9, N13, N14 and X may be missing
Expression Markers	Negative for estrogen receptor, progesterone receptor and HER2 (triple-negative); expresses an exon-5-deleted estrogen receptor mRNA variant. HLA-A1 and Bw16 (+/-)
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically takes 2 to 5 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium, and gently pipette to prepare a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	A split ratio of 1:2 to 1:4 is recommended.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO

Cat.No.	Product Name	Price
CSC-RR01023	GFP/Luc Reporter Cell Line - BT-20	Inquiry

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* For research use only. Not intended for any clinical use.

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