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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | Caki-1 is a human renal cell carcinoma cell line derived from a cutaneous metastasis in a 49-year-old Caucasian male patient with clear cell renal cell carcinoma (ccRCC). Caki-1 cells exhibit an epithelial morphology and grow as an adherent monolayer under standard culture conditions. The cell line retains distinct ultrastructural characteristics of proximal renal tubular epithelial cells, such as microvilli, multilayered cellular lamellae, and microfilaments. Cytogenetically, Caki-1 displays an aneuploid karyotype with complex chromosomal alterations. At the molecular level, Caki-1 expresses wild-type VHL, making it a valuable model for comparative studies with VHL-mutant renal cancer cell lines. Furthermore, the cell line demonstrates high tumorigenicity and metastatic potential in vivo. Due to its well-defined phenotypic and physiological properties, Caki-1 is widely used as an in vitro model to investigate the biology of clear cell renal carcinoma, explore angiogenesis and hypoxia pathways, evaluate anti-angiogenic drugs and targeted therapies, and conduct functional genomic screens. |
| Tissue | Kidney |
| Disease | Clear Cell Renal Cell Carcinoma (ccRCC); Kidney Cancer |
| Morphology | Epithelial |
| Gender | Male |
| Age | 49 years |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Research on the biological characteristics and metastatic progression of clear cell renal cell carcinoma (ccRCC) 2. In vitro evaluation of targeted therapies, tyrosine kinase inhibitors (TKIs), and anti-angiogenic drugs 3. Comparative study of signaling pathways and hypoxic responses in VHL wild-type versus VHL-mutant cells 4. Functional genomic analysis, target identification, and CRISPR/Cas9 knockout screening |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid; modal chromosome number is triploid |
| Tumorigenic | Yes, forms tumors in immunocompromised (nude) mice |
| Genetic Profile | VHL wild-type; TP53 wild-type / altered pathways; MET overexpression |
| Expression Markers | Expresses proximal tubular epithelial markers; positive for cytokeratins and renal cell carcinoma antigen |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (without Ca2+/Mg2+) to completely remove residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.03% EDTA solution and incubate at 37°C until the cell monolayer detaches (typically 2 to 5 minutes). 4. Add fresh complete growth medium to neutralize the trypsin and gently pipette up and down to prepare a single-cell suspension. 5. Subculture the cell suspension into new culture vessels. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | A split ratio of 1:3 to 1:6 is recommended |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO02550 | Human ANXA1 Stable Cell Line - Caki-1 | Inquiry |
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