SW13 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	SW13 is a human adrenocortical carcinoma cell line established by A. Leibovitz and his colleagues in 1971. This cell line was isolated from the primary tumor tissue of a 55-year-old female patient diagnosed with small-cell adrenocortical carcinoma. SW13 cells exhibit a small, epithelial-like morphology and grow as adherent monolayers. This cell line holds immense significance in the fields of modern oncology and cell biology, as it is widely recognized as the global "gold standard" model for studying defects in the SWI/SNF chromatin remodeling complex. Due to its inherent deficiency in the core subunits of the SWI/SNF complex—specifically the proteins BRG1 (SMARCA4) and BRM (SMARCA2)—SW13 cells are extensively utilized to investigate epigenetic gene regulation, defects in steroid biosynthesis, and viral replication mechanisms (such as the proliferation processes of influenza viruses, polyomaviruses, and flaviviruses).
Tissue	Adrenal cortex
Disease	Adrenal Cortex Carcinoma / Small-Cell Carcinoma
Morphology	Small, epithelial-like
Gender	Female
Age	55 years
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. The "Gold Standard" model for studying SWI/SNF subunit-deficient cancers and epigenetic gene regulation 2. Elucidating the dynamics of chromatin remodeling during cellular transcription and differentiation cascades 3. Profiling of host-virus interactions, particularly for viruses dependent on specific cellular machinery 4. Preclinical screening of novel therapeutic agents and kinase inhibitors for adrenocortical carcinoma 5. Analysis of tumor initiation and metastasis processes using xenograft models
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase)
Characteristics
Tumorigenic	Yes, reliably forms small-cell carcinomas when engrafted into immunocompromised mice (e.g., nude or SCID mice)
Karyotype	Aneuploid / Hyperdiploid; a highly rearranged human karyotype with a modal chromosome number fluctuating between 48 and 54.
Genetic Profile	1. BRG1 (SMARCA4) Deficiency: Exhibits a constitutive state of deficiency resulting from genomic mutation or silencing. 2. BRM (SMARCA2) Deficiency: Presents as a concomitant deficiency, thereby establishing a rare "double-negative" SWI/SNF core status. 3. Vimentin Expression: Displays cellular heterogeneity, manifesting as distinct vimentin-positive and vimentin-negative subclones, both of which exhibit divergent structural dynamic characteristics.
Growth Kinetics	Highly proliferative and dense monolayer expansion; doubling time typically ranges between 24 to 34 hours.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Completely remove and discard the spent culture medium. 2. Gently rinse the confluent cell monolayer with Ca²⁺/Mg²⁺-free Dulbecco's Phosphate-Buffered Saline (DPBS) to neutralize residual serum components. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, ensuring the cell layer is completely submerged. 4. Incubate at 37°C for 2 to 6 minutes; observe under an inverted microscope until the cell layer has completely detached and the cells have rounded up. 5. Add an equal or greater volume of complete culture medium to thoroughly neutralize the trypsin activity. 6. Centrifuge at approximately 200-300 × g for 5 minutes; discard the supernatant, gently resuspend the cell pellet in fresh medium, and aliquot evenly into new culture flasks.
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	A split ratio of 1:3 to 1:6 is standardly recommended for routine maintenance.
Culture Conditions	Atmosphere: Air, 100% (If using L-15 Medium) OR Air, 95%,CO2, 5% (If using DMEM); Temperature: 37°C
Cryopreservation	90% Complete growth medium + 10% DMSO (or 50% Basal medium + 40% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RO02576	Cas9 Stable Cell Line - SW13	Inquiry
CSC-RR00878	GFP Reporter Cell Line - SW-13	Inquiry
CSC-RR00927	Luciferase Reporter Cell Line - SW-13	Inquiry

The above is only part of a part of cell line products. If you don't find the cell line you want, Creative Biogene can also provide stable cell line generation service with the best prices and fastest turnaround time for you! Contact us for more information or to request a quote.

* For research use only. Not intended for any clinical use.

Quick Inquiry