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MicroRNA Agomir Antagomir Synthesis Service


MicroRNA Agomir Antagomir Synthesis Service

  1. How to do the conversion between OD and nmol of RNA oligo?
  2. How do I calculate the amount of buffer needed to resuspend siRNA if I need a stock concentration of 20 µM?
  3. What should be provided for microRNA agomir/ antagomir synthesis service?
  4. What type of modification was made for agomir/ antagomir?

1.  How to do the conversion between OD and nmol of RNA oligo?

There is a precise formula for the conversion between OD and nmol of agomir/ antagomir, In general, there is a simple calculation as follows.

Agomir: 1 OD duplex=2.5 nmol = 33µg

Antagomir: 1 OD duplex=5 nmol = 33µg


2.  How do I calculate the amount of buffer needed to resuspend siRNA if I need a stock concentration of 20 µM?

Agomir/ Antagomir from Creative Biogene is supplied as a dried pellet in nmol quantities. Dissolve miRNA antagomir to a convenient stock concentration using the recommended volume of DEPC H2O (or RNase-free water). For example: Dissolve 1 OD miRNA antagomir to 20 µM using 250 µl DEPC H2O (or RNase-free water).

Dissolve miRNA agomir to a convenient stock concentration using the recommended volume of DEPC H2O (or RNase-free water). For example: Dissolve 1 OD miRNA agomir to 20 µM using 125 µl DEPC H2O (or RNase-free water).


3.  What should be provided for microRNA agomir/ antagomir synthesis service?

To synthesis your microRNA agomir/antagomir, you just need to provide the following information:

  1. The name and species of the mature microRNA or the accession number of it or the mature microRNA sequence.
  2. The quantity you need to synthesis.

4.  What type of modification was made for agomir/ antagomir?

The Chemically-modified Construction of Antagomir and Agomir:

  1. Antogimir:
    3' terminal was modified by cholesterol, there are two thiols modification in the 5' terminal, four thiols modification in the 3' terminal, and the whole strand is modified by 2'-OMe.
  2. Agomir:
    in the antisense strand, 3' terminal was modified by cholesterol, there are two thiols modification in the 5' terminal, four thiols modification in the 3'terminal, and the whole strand is modified by OMe.

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