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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | OCI-AML-3 (also known as OCI-AML3) is a human acute myeloid leukemia (AML) cell line established in 1987 by the Ontario Cancer Institute from the peripheral blood of a 57-year-old male patient at the time of diagnosis. The disease is classified as acute myeloid monocytic leukemia (AML FAB M4). OCI-AML-3 plays a crucial role in hematologic oncology and is the gold standard in vitro model of NPM1-mutant AML (the most common molecular subtype of adult AML). It retains key phenotypic features, including abnormal localization of mutant nucleolar phosphatase in the cytoplasm and lack of CD34 expression. It is widely used globally to study leukemia development, clonal hematopoiesis, epigenetic dysregulation, and to evaluate emerging therapies such as menin inhibitors and targeted chemotherapy. |
| Tissue | Peripheral blood |
| Disease | Acute Myeloid Leukemia (AML FAB M4; Myelomonocytic) |
| Morphology | Monoblast-like / Lymphoblast-like; single, mostly round cells |
| Gender | Male |
| Age | 57 years |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Molecular pathobiology of NPM1-mutated and DNMT3A-mutated acute myeloid leukemia 2. Epigenetic studies focusing on DNA methylation pathways 3. Evaluation of targeted therapies (e.g., menin-KMT2A inhibitors, BCL-2 inhibitors such as venetoclax) 4. Preclinical high-throughput drug screening of antileukemia compounds 5. Detection of apoptosis and differentiation responses |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, reliably engrafts and models AML in immunocompromised mice (e.g., NOD/SCID) |
| Karyotype | Human hyperdiploid karyotype; modal number = 48 (range 45-50) |
| Genetic Profile | 1. NPM1 gene mutation: Heterozygous type A mutation 2. DNMT3A gene mutation: Heterozygous hotspot mutation 3. NRAS gene mutation: Homozygous mutation 4. FLT3 status: FLT3 wild type |
| Immunophenotype | Positive for CD4, CD13, CD14, CD15, cyCD68; Negative for CD3, CD19, HLA-DR; Weakly positive/negative for CD33 and CD34 |
| Growth Kinetics | The doubling time is approximately 30 to 40 hours; the maximum density is approximately 2.5 × 10⁶ cells/mL. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Suspension cells do not require trypsin digestion. Collect the cell suspension completely from the culture flask. 2. Centrifuge at 1000 rpm (approximately 200-300 × g) for 5 minutes. 3. Aspirate the supernatant completely, ensuring the cell pellet remains stationary. 4. Resuspend the cells in fresh, preheated complete culture medium. 5. Seed at a density of 0.3 × 10⁶ to 0.5 × 10⁶ cells/mL. Strictly maintain the working culture density between 0.5 × 10⁶ and 2.0 × 10⁶ cells/mL. |
| Medium Renewal | Every 2 to 3 days (depending on cellular density and pH of the medium) |
| Subcultivation Ratio | Split saturated cultures at a ratio of 1:3 to 1:4 |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 70% to 80% Alpha-MEM + 10% to 20% FBS + 10% DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0584 | GFP Reporter Cell Line - OCI-AML-3 | Inquiry |
| CSC-RR0585 | Luciferase Reporter Cell Line - OCI-AML-3 | Inquiry |
| CSC-RR0586 | GFP/Luc Reporter Cell Line - OCI-AML-3 | Inquiry |
| CSC-RO01212 | Cas9 Stable Cell Line - OCI-Aml-3 | Inquiry |
| CSC-RO01630 | OVAL Stable Cell Line - OCI-AML-3 | Inquiry |
| CSC-RO01883 | GFP/OVAL Stable Cell Line - OCI-AML-3 | Inquiry |
| CSC-RO02136 | Luc/OVAL Stable Cell Line - OCI-AML-3 | Inquiry |
| CSC-RO02389 | GFP/Luc/OVAL Stable Cell Line - OCI-AML-3 | Inquiry |
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