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| General Information | |
| Organism | Mus musculus, mouse |
| Cell Line Description | L929 (also known as L-929 or Clone 929) is the first continuous cell line ever established in the world. It is a clonal derivative of the L cell line, which was isolated in 1948 by Earle and his colleagues from the subcutaneous connective tissue of a male C3H/An mouse. L929 exhibits a typical fibroblast morphology, grows rapidly, and is easy to culture. It stands as one of the most classic cellular models in the fields of toxicology, pharmacology, and virology. Due to its extreme sensitivity to Tumor Necrosis Factor (TNF) and its manifestation of specific necroptosis characteristics, it is widely utilized for detecting TNF activity, investigating the mechanisms of programmed cell death, and evaluating the biocompatibility of medical devices. |
| Tissue | Subcutaneous connective tissue; areolar and adipose |
| Cell Type | Fibroblast |
| Morphology | Fibroblast-like |
| Gender | Male |
| Age | 100 days |
| Strain | C3H/An |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Biocompatibility assessment of medical devices 2. Quantitative determination of the bioactivity of tumor necrosis factor (TNF) 3. Investigation into the molecular mechanisms of programmed necrosis (necroptosis) 4. Bioassays of interferon and studies on viral replication 5. Studies on the cell cycle, collagen synthesis, and metabolism in basic cell biology |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | Yes, in syngeneic C3H mice or immunocompromised mice |
| Karyotype | Heteroploid; the chromosome number typically ranges between 60 and 70 (diploid mice: 2n=40). |
| Sensitivity | Extremely sensitive to TNF-α-induced cell death (particularly in the presence of Actinomycin D). |
| Growth Kinetics | The doubling time is approximately 21–24 hours. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Discard the old culture medium. 2. Wash the cell monolayer with Ca²⁺/Mg²⁺-free PBS. 3. Add 0.25% Trypsin – 0.53 mM EDTA, and incubate at room temperature or 37°C for 2–5 minutes until the cells detach. 4. Add complete medium containing serum to stop the digestion, and gently pipette to mix thoroughly. 5. Split the cells into new flasks at the appropriate ratio. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | 1:3 to 1:8 |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete growth medium + 10% DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO1146 | Human FAP Stable Cell Line - L929 | Inquiry |
| CSC-RO01196 | Cas9 Stable Cell Line - L-929 | Inquiry |
| CSC-RR00688 | Luciferase Reporter Cell Line - L-929 | Inquiry |
| CSC-RR00790 | GFP Reporter Cell Line - L929 | Inquiry |
| CSC-RR01203 | GFP/Luc Reporter Cell Line - L929 | Inquiry |
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