Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Premade Virus Particles
Ready-to-Use | High Titer | Versatile Applications
Premade AAV, adenovirus, lentivirus particles, safe, stable, in stock.
Virus-Like Particles (VLPs)
Stable | Scalable | Customizable
Advanced VLPs for vaccine development (Chikungunya, Dengue, SARS-CoV-2), gene therapy (AAV1 & AAV9), and drug screening (SSTR2, CCR5).
Oligonucleotide Products
Precise | High Yield | Tailored Solutions
Accelerate your research with cost-effective LncRNA qPCR Array Technology.
RNA Interference Products
Targeted | Potent | High Specificity
Human Druggable Genome siRNA Library enables efficient drug target screening.
Recombinant Drug Target Proteins
Authentic | Versatile | Accelerated
Providing functional, high-purity recombinant proteins—including membrane proteins and nanodiscs—to overcome bottlenecks in drug screening and target validation.
Clones
Validated | Reliable | Comprehensive Collection
Ready-to-use clones for streamlined research and development.
Kits
Complete | Convenient | High Sensitivity
Chromogenic LAL Endotoxin Assay Kit ensures precise, FDA-compliant endotoxin quantification for biosafety testing.
Enzymes
Purified | Stable | Efficient
Powerful Tn5 Transposase for DNA insertion and random library construction.
Aptamers
Highly Specific | Robust | Versatile
Aptamers for key proteins like ACVR1A, Akt, EGFR, and VEGFR.
Adjuvants
Enhancing | Synergistic | Effective
Enhance immune responses with high-purity, potent CpG ODNs.
Laboratory Equipment
Innovative | Reliable | High-Precision
Effortlessly streamline DNA extraction with CB™ Magnetic-Nanoparticle Systems.
Stable Cell Line Generation
Reliable | Scalable | Customizable
Fast proposals, regular updates, and detailed reports; strict quality control, and contamination-free cells; knockout results in 4-6 weeks.
Target-based Drug Discovery Service
Innovative | Comprehensive | Efficient
Target identification, validation, and screening for drug discovery and therapeutic development.
Custom Viral Service
Versatile | High-Yield | Safe
Unbeatable pricing, fully customizable viral packaging services (covering 30,000+ human genes, 200+ mammals, 50+ protein tags).
Custom Antibody Service
Precise | Flexible | Efficient
End-to-end antibody development support, from target to validation, enabling clients to rapidly obtain application-ready antibodies.
Antibody-Drug Conjugation Service
Integrated | Controlled | Translational
Comprehensive solutions covering design, development, and validation to ensure conjugated drugs with consistent quality and clinical potential.
Protein Degrader Service
Efficient | High-Precision | Advanced Therapeutics
Harness the power of protein degraders for precise protein degradation, expanding druggable targets and enhancing therapeutic effectiveness for cutting-edge drug discovery.
Nucleotides Service
Accurate | Flexible | High-Quality
Custom synthesis of oligonucleotides, primers, and probes for gene editing, PCR, and RNA studies.
Custom RNA Service
Custom RNA ServicePrecise | Flexible | GMP-ReadyCustom
RNA design, synthesis, and manufacturing—covering mRNA, saRNA, circRNA, and RNAi. Fast turnaround, rigorous QC, and seamless transition from research to GMP production.
Custom Libraries Construction Service
Comprehensive | High-throughput | Accurate
Custom cDNA, genomic, and mutagenesis libraries for drug discovery, screening, and functional genomics.
Gene Editing Services
Precise | Efficient | Targeted
Gene editing solutions for gene editing, knockouts, knock-ins, and customized genetic modifications. Integrated multi-platform solutions for one-stop CRISPR sgRNA library synthesis and gene screening services
Microbe Genome Editing Service
Precise | Scalable | Customizable
Enhance microbial productivity with advanced genome editing using Rec-mediated recombination and CRISPR/Cas9 technologies.
Biosafety Testing Service
Reliable | Comprehensive | Regulated
Complete biosafety testing solutions for gene therapy, viral vectors, and biologics development.
Plant Genetic Modification Service
Advanced | Sustainable | Tailored
Genetic modification for crop improvement, biotechnology, and plant-based research solutions.
Plant-based Protein Production Service
Efficient | Scalable | Customizable
Plant-based protein expression systems for biopharmaceuticals, enzyme production, and research.
Aptamers Service
Innovative | Fast | Cost-Effective
Revolutionizing drug delivery and diagnostic development with next-generation high-throughput aptamer selection and synthesis technologies.
CGT Biosafety Testing
Comprehensive | Accurate | Regulatory-compliant
Internationally certified evaluation system for biologics, gene therapies, nucleic acid drugs, and vaccines.
Pandemic Detection Solutions
Rapid | Precise | Scalable
Balancing accuracy, accessibility, affordability, and rapid detection to safeguard public health and strengthen global response to infectious diseases.
cGMP Cell Line Development
Reliable | Scalable | Industry-leading
Stable expression over 15 generations with rapid cell line development in just 3 months.
Supports adherent and suspension cell lines, offering MCB, WCB, and PCB establishment.
GMP mRNA Production
Efficient | Scalable | Precise
Scalable mRNA production from milligrams to grams, with personalized process design for sequence optimization, cap selection, and nucleotide modifications, all in one service.
GMP Plasmid Production
High Quality | Scalable | Regulatory-compliant
Our plasmid production services span Non-GMP, GMP-Like, and GMP-Grade levels, with specialized options for linearized plasmids.
GMP Viral Vector Manufacturing
Scalable | High Yield | Quality-driven
Advanced platforms for AAV, adenovirus, lentivirus, and retrovirus production, with strict adherence to GMP guidelines and robust quality control.
AI-Driven Gene Editing and Therapy
Innovative | Precision | Transformative
AI-powered one-click design for customized CRISPR gene editing strategy development.
AI-Antibody Engineering Fusion
Next-Generation | Targeted | Efficient
AI and ML algorithms accelerate antibody screening and predict new structures, unlocking unprecedented possibilities in antibody engineering.
AI-Driven Enzyme Engineering
Smart | Efficient | Tailored
High-throughput enzyme activity testing with proprietary datasets and deep learning models for standardized and precise enzyme engineering design.
AI-Enhanced Small Molecule Screening
Predictive | Efficient | Insightful
Leverage AI to uncover hidden high-potential small molecules, prioritize leads intelligently, and reduce costly trial-and-error in early drug discovery.
AI-Driven Protein Degrader Drug Development
Innovative | Targeted | Accelerated
Use AI-guided design to optimize protein degraders, addressing design complexity and enhancing efficacy while shortening development timelines.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
RNAi is a post-transcriptional mechanism that involves the inhibition of gene expression through promoting cleavage on a specific area of a target messenger RNA (mRNA). This technology has shown promising therapeutic results for a good number of diseases, especially in cancer.
RNA interference (RNAi), a process that involves sequence-specific gene silencing, is an endogenous post-transcriptional regulation process that consists of non-coding RNAs like microRNAs (miRNAs), small interfering RNAs (siRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs), which target and silence messenger RNAs (mRNAs) in a sequence-specific manner. With the added benefit of simultaneously targeting multiple genes that share homology, RNAi is fast, economical, and has the potential for site-specific targeting. The primary role of RNAi in cells would be to downregulate the expression of their target gene and thereby the encoded proteins thus bringing about the desired results. Through careful sequence selection and synthesis of tailored non-coding RNAs (ncRNAs), this powerful approach could be used to circumvent conventional cancer therapy limitations, thus paving the way for next generation therapeutics.
Fig. 1 Schematic illustration of RNAi mechanism.
Given its target site specificity and therapeutic potential, RNAi is currently used in many research fields. The following parts will briefly describe some applications of RNAi in biomedicine.
| Typical Disease Fields | ||
| Neurodegenerative Disease | Cardiovascular Disease | Viral Infection |
| Lung Cancer | Pancreatic Cancer | Breast Cancer |
| Colorectal Cancer | Other Cancers | |
The only RNAi drug on the market is called rintatolimod, with the United States trade name Ampligen. Rintatolimod is a double-stranded RNA drug first launched in Argentina in 2017 for the treatment of severe myalgic encephalomyelitis/chronic fatigue syndrome. Currently, some progress has been made in preclinical studies. Phase I and I/II clinical trials are ongoing at AIM ImmunoTech (formerly HemispheRx) to evaluate its vaccine adjuvant potential for the treatment of stage II–IV HER2+ breast cancer, triple-negative breast cancer, and several other solid tumors, such as renal cell carcinoma, pancreatic cancer, and ovarian cancer. The molecular mechanism is Toll-Like Receptor 3 agonists, and it was found that the activation of TLR3 in vitro can induce apoptosis in lung cancer cell lines. In addition, some studies support the use of TLR3 agonists in patients with non-small cell lung cancer (NSCLC) to reactivate the local innate immune response.
Genasense (oblimersen) is an antisense oligonucleotide drug specific to Bcl-2. Genasense can specifically bind to human Bcl-2 mRNA, which leads to the catalytic degradation of Bcl-2 mRNA and reduces the translation of Bcl-2 protein. Therefore, researchers can use mRNA degradation strategies and small inhibitory molecules for targeted Bcl-2 therapy in a variety of cancers, such as breast cancer, colon cancer, and prostate cancer.
Since the first RNAi drug was launched, some RNAi drugs (such as HBV-ISS, mipomersen sodium, nusinesen, inotersen, volanesorsen, patisiran/onpattro, pegaptanib sodium, and viltolarsen) have been used in clinical treatment and have shown unparalleled advantages over other types of drugs.
Great efforts have been made to design and develop various carriers to overcome the obstacles in the delivery process. In general, these carriers can be divided into two categories: viral vectors and non-viral vectors.
Various lipid-based siRNA delivery systems have been reported, including liposomes, solid LNP, micelles, and emulsions. In fact, LNP is considered a popular vector because of its good stability and high transfection efficiency.
Fig. 2 (A) Liposome. (B). Formation and secretion of exosomes.
Many types of cationic polymers have been studied as carriers; one of the types is synthetic polymer (polycation containing cyclodextrin (CD), PEI, etc.) and the other is natural polymer (chitosan).
To date, cancer remains the leading cause of death in humans, further uncontrolled proliferation and aggressiveness of cancer cells render many medical treatments ineffective. The remarkable gene silencing capabilities of siRNA therapy offer a glimpse into the future, offering new strategies for the treatment of cancer and other diseases.
References:
Copyright © Creative Biogene. All rights reserved.