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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | KYSE-520 is a human esophageal squamous cell carcinoma (ESCC) cell line derived from an invasive, moderately differentiated tumor located in the lower thoracic esophagus of a 58-year-old Japanese female; the primary tumor had invaded the adventitia. As a member of the well-characterized KYSE series of esophageal cancer cell lines, KYSE-520 exhibits an epithelioid morphology and grows as an adherent monolayer, with giant cells occasionally observed within the population (accounting for approximately 1%). Genetically, this cell line harbors a splice acceptor site mutation (c.376-2A>T) in the tumor suppressor gene TP53, alongside an amplification of the oncogene MYC—both of which are frequently reported alterations in esophageal squamous cell carcinoma. KYSE-520 expresses various squamous and epithelial markers, including pan-cytokeratin, cytokeratins 8, 17, 18, and 19, as well as EpCAM and vimentin; however, it does not express cytokeratin 7, desmin, glial fibrillary acidic protein (GFAP), or neurofilament. This cell line serves as a practical platform for investigating the mechanisms of squamous cell carcinogenesis, p53-dependent signaling pathways, tumor radiosensitivity, and chemotherapy resistance, as well as for evaluating potential therapeutic agents. Given its well-defined genetic background, KYSE-520 is widely utilized in both monolayer cell assays and animal models of esophageal cancer, playing a significant role in target validation and biomarker discovery. |
| Tissue | Esophagus; lower intra-thoracic esophagus (primary tumor, resected prior to treatment) |
| Disease | Esophageal Squamous Cell Carcinoma (ESCC); Moderately differentiated invasive |
| Morphology | Epithelial-like (epithelioid); occasional giant cells present (ca. 1%) |
| Gender | Female |
| Age | 58 years |
| Ethnicity | Japanese |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Investigation of esophageal squamous cell carcinoma (ESCC) biology and squamous cell carcinogenesis 2. Analysis of TP53-dependent DNA damage response, apoptosis and cell cycle control 3. Study of MYC oncogene amplification and MYC-driven transcriptional programs 4. Evaluation of radiation sensitivity, chemoresistance and combination therapy strategies 5. Screening of small-molecule inhibitors and receptor-targeted agents, including EGFR- and FGFR-directed agents 6. Development of subcutaneous and orthotopic xenograft models in immunodeficient mice |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Doubling Time | Approximately 30 hours |
| Tumorigenic | Yes, in immunodeficient (nude / NOD-SCID) mice |
| Karyotype | Human flat-moded hypotriploid karyotype with 8% polyploidy; modal chromosome number = 58-60 (XX, loss of one X) |
| Expression Markers | Positive for pan-cytokeratin, cytokeratin 8, cytokeratin 17, cytokeratin 18, cytokeratin 19, EpCAM and vimentin; negative for cytokeratin 7, desmin, GFAP and neurofilament |
| Mycoplasma Test | Negative |
| Viral Status | Reverse transcriptase negative; PCR-negative for EBV, HBV, HCV, HHV-8, HIV-1, HIV-2, HPV, HTLV-1/2, MLV and SMRV |
| Culture Conditions and Handling | |
| Subculturing | 1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically takes 2 to 5 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium, and gently pipette to prepare a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | When seeding new culture vessels, the cell density should be approximately 0.5-1.0 X 10⁶ cells per 80 cm². Once the cells reach confluence, they should be subcultured using trypsin/EDTA at a split ratio of 1:3 to 1:6, with subculturing performed every 3–4 days (yielding approximately 4-5 X 10⁶ cells per 80 cm²). |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 10% (v/v) DMSO; alternatively, 70% complete growth medium + 20% FBS + 10% DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0344 | GFP Reporter Cell Line - KYSE-520 | Inquiry |
| CSC-RO01502 | OVAL Stable Cell Line - KYSE-520 | Inquiry |
| CSC-RO01755 | GFP/OVAL Stable Cell Line - KYSE-520 | Inquiry |
| CSC-RO02008 | Luc/OVAL Stable Cell Line - KYSE-520 | Inquiry |
| CSC-RO02261 | GFP/Luc/OVAL Stable Cell Line - KYSE-520 | Inquiry |
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