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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | MFE-280 is a human endometrial adenocarcinoma cell line established from recurrent tumor tissue. The donor tumor was described as adenomatous, partly papillary, and high grade (G3). The cells grow as an adherent epithelial-like monolayer and have a relatively slow reported doubling time. Documented progesterone receptor expression, placental protein 14 expression, microsatellite stability, and defined PIK3CA and TP53 variants make this model useful for mechanistic and translational studies of high-grade endometrial cancer, hormone-related biology, oncogenic signaling, and therapeutic response. |
| Cell Type | Human endometrial adenocarcinoma cell |
| Tissue of Origin | Endometrium |
| Disease | Recurrent endometrial adenocarcinoma; adenomatous, partly papillary, grade G3 |
| Sex | Female |
| Age at Sampling | 77 years |
| Population | Caucasian |
| Morphology | Epithelial-like (epithelioid) |
| Growth Mode | Adherent monolayer |
| Product Format | Frozen |
| Biosafety Level | Biosafety Level 1 under the referenced classification. |
| Applications | 1. Endometrial adenocarcinoma biology 2. High-grade and recurrent tumor models 3. Progesterone receptor and hormone-response studies 4. Placental protein 14 / glycodelin expression research 5. PI3K-pathway studies associated with PIK3CA alteration 6. TP53 dysfunction and genome-stability research 7. Microsatellite-stable endometrial cancer models 8. Anticancer drug-response and resistance studies 9. Xenograft and tumorigenicity research 10. Genomic, transcriptomic, proteomic, epigenomic, and functional-screening studies |
| Characteristics | |
| Tumor Histology | Derived from a recurrent endometrial carcinoma described as adenomatous, partly papillary, and grade G3 |
| Hormone Receptor | Progesterone receptor expression has been reported |
| PIK3CA Variant | Heterozygous PIK3CA c.3139C>T, resulting in p.His1047Tyr |
| TP53 Variant | Homozygous TP53 c.919+1G>C splice-donor variant |
| Microsatellite Status | Microsatellite stable (MSS) |
| Karyotype | A hyperdiploid human karyotype with a modal chromosome count near 52 and approximately 24% polyploidy has been reported. The documented profile includes multiple numerical and structural abnormalities, including gain of chromosome arm 1q. |
| Tumorigenicity | Reported to form heterotransplantable tumors in immunodeficient mice |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Culture Conditions | 37°C in a humidified atmosphere containing 5% CO₂ |
| Recommended Seeding Density | Seed 2–6 × 10⁶ cells per 75 cm² culture flask |
| Passage Endpoint | Subculture when the monolayer is confluent |
| Subcultivation Ratio | Split at a ratio of 1:2 to 1:6 every 3–5 days, using trypsin/EDTA for detachment |
| Subculturing | 1. Remove the spent medium from a confluent culture. 2. Detach the monolayer with trypsin/EDTA while monitoring the cells microscopically. 3. Add complete growth medium to stop dissociation and collect the cell suspension. 4. Resuspend gently to obtain an even suspension. 5. Seed fresh culture vessels at a 1:2 to 1:6 split ratio, maintaining the reported inoculum range where possible. 6. Return the cultures to 37°C and 5% CO₂ and monitor attachment and growth. |
| Post-Thaw Recovery | Seed the thawed suspension into a 75 cm² flask using complete medium. Approximately 4 hours after seeding, transfer the medium and floating cells into a second flask rather than discarding them; this preserves the initially non-adherent fraction described in the referenced recovery procedure. Continue both cultures under the standard incubation conditions. |
| Cryopreservation Medium | 70% complete culture medium + 20% fetal bovine serum + 10% DMSO |
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