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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | PC-3M is a highly metastatic, adherent human prostate cancer cell line established in 1984 by J.M. Kozlowski and colleagues. It was isolated as a subline selected in vivo from liver metastases in nude mice after intrasplenic injection of a parental PC-3 cell line (originally derived from bone metastases in a 62-year-old white male). PC-3M cells grow as adherent monolayers of epithelial-like cells. PC-3M is globally recognized as the premier model for treating advanced invasive prostate cancer, exhibiting significantly increased invasiveness and metastatic potential compared to parental PC-3 cells. It is extensively used in cancer research to investigate tumor cell migration, extravasation, epithelial-mesenchymal transition (EMT), and to screen anti-metastatic therapies. |
| Tissue | Prostate; derived from metastatic site (liver metastasis via parental bone metastasis lineage) |
| Cell Type | Epithelial cell |
| Disease | Prostate Adenocarcinoma / Prostate Cancer |
| Morphology | Epithelial-like |
| Gender | Male |
| Age | 62 years (age of the original PC-3 donor) |
| Product Format | Frozen |
| Growth Mode | Adherent Monolayer |
| Biosafety Level | 1 (Biosafety classification aligns with global standard laboratory parameters for non-infectious human tumor cells) |
| Applications | 1. In vitro and in vivo gold standard models for studying prostate cancer invasion, migration, and organ-specific metastasis. 2. Construction of orthotopic or systemic metastasis models in immunodeficient mice to study tumor colonization. 3. Elucidation of molecular pathways in the progression of androgen-independent prostate cancer. 4. Preclinical high-throughput screening of anti-invasive drugs, small molecule kinase inhibitors, and therapeutic targets. 5. Study of extracellular matrix degradation, matrix metalloproteinase (MMP) activity, and tumor angiogenesis. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C (Liquid nitrogen vapor phase only) |
| Characteristics | |
| Tumorigenic | Yes, highly tumorigenic and highly metastatic in nude mice, with a strong propensity to colonize the liver, lungs, and lymph nodes. |
| Lineage Relationship | Derivative subline of the parental PC-3 cell line, selected through in vivo metastatic enrichment. |
| Hormone Receptor Status | Androgen Receptor (AR) negative and Prostate-Specific Antigen (PSA) negative, representing an advanced, androgen-independent (castration-resistant) state. |
| Genetic Profile | 1. TP53 status: Hemizygous deletion/mutation (consistent with parental PC-3). 2. PTEN status: Deletion (homozygous deletion, leading to overactivation of the PI3K/Akt signaling pathway). |
| Growth Kinetics | Highly proliferative with rapid cell cycle progression; typical population doubling time spans approximately 24 to 30 hours. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Completely remove and discard the used complete culture medium. 2. Gently wash the cell monolayer with sterile PBS (Ca2+/Mg2+-free) to remove any residual serum. 3. Add 1.0 to 2.0 mL of 0.25% trypsin-0.53 mM (0.03%) EDTA solution to completely cover the cell layer. 4. Incubate at room temperature or 37°C for 2 to 5 minutes; observe under an inverted microscope until the cells become rounded, loose, and completely detached. 5. Add an equal volume of complete culture medium to rapidly neutralize the trypsin activity. 6. Centrifuge the cell suspension at approximately 125 × g for 5 minutes. Carefully aspirate the supernatant, gently resuspend the pellet in fresh complete culture medium, and aliquot into new containers. |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | Split confluent cultures at a standard ratio of 1:3 to 1:8. Do not allow the cells to remain at 100% confluence for prolonged periods to prevent cell piling and shedding. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete culture growth medium + 10% DMSO (or 50% RPMI 1640 + 40% FBS + 10% DMSO) |
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