RSC96 Cell Line

Brief Introduction
Product List

General Information
Organism	Rattus norvegicus, rat
Cell Line Description	RSC96 is a rat Schwann cell line established through spontaneous immortalization. Developed by A. Badache and G. H. De Vries, the line originated from primary Schwann cells isolated from the rat peripheral nervous system, which were subsequently subjected to long-term culture and continuous passaging. RSC96 cells exhibit an elongated spindle-like morphology (typically bipolar or tripolar) and grow as an anchorage-dependent monolayer. The cell line is widely recognized as a fundamental in vitro model in the fields of neurobiology and peripheral nerve repair research. By overcoming the contamination issues and limited lifespan often associated with primary cell isolation, RSC96 has become an indispensable alternative model for studying Schwann cell proliferation, migration mechanisms, gene pathways related to myelination, and scaffold materials for peripheral nerve injury regeneration.
Tissue	Peripheral Nervous System; Peripheral Nerve
Cell Type	Glial cell; Schwann cell
Disease	Normal / Non-malignant tissue origin (Spontaneously immortalized)
Morphology	Elongated; spindle-shaped with fine bipolar or tripolar processes
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification aligns with global laboratory safety directives for non-infectious animal materials)
Applications	1. Directly establish in vitro models to profile the transcription of Schwann cell adhesion, migration, and myelination; 2. Evaluate the biocompatibility, axonal guidance effects, and tissue regeneration dynamics of biomaterial scaffolds; 3. Elucidate signal transduction networks driven by growth factors such as platelet-derived growth factor (e.g., PDGF-BB); 4. Investigate toxicity cascades, oxidative stress pathways, and drug-induced peripheral neuropathy; 5. Conduct functional genetic assessments using targeted gene knockout models (e.g., L-periaxin knockout studies).
Shipped In	Dry ice
Storage Temperature	−196°C (Liquid nitrogen vapor phase only)
Characteristics
Tumorigenic	No, non-tumorigenic under standard physiological environments.
Transformant	None; immortalized via continuous spontaneous replication adaptation during extended selection in culture.
Expression Profile	These cells naturally express high levels of functional platelet-derived growth factor (PDGF) receptors and undergo an active, dose-dependent cell division cascade upon exposure to exogenous PDGF-BB; they serve as a functional platform for studying peripheral cell adhesion and the dynamic structural changes of focal adhesions.
Genetic/Lineage Status	Authenticated as a pure Rattus norvegicus cellular baseline via continuous species-specific PCR profiling. It serves as the parental lineage for custom genetic lines, including TALEN-mediated L-periaxin knockout variations.
Growth Kinetics	Moderate to rapid adherent expansion; population doubling time spans approximately 30 to 45 hours.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the spent culture medium from the culture flask. 2. Briefly rinse the cell monolayer with 0.25% trypsin–0.53 mM (0.03%) EDTA solution to remove residual serum containing trypsin inhibitors thoroughly. 3. Add 2.0 to 3.0 mL of trypsin–EDTA solution to cover the cell layer. 4. Incubate at room temperature or 37°C for 5 to 15 minutes; monitor under an inverted microscope until the cell layer detaches, rounds up, and completely disperses. 5. Critical Clumping Warning: To prevent permanent cell aggregation, do not tap, shake, or otherwise agitate the flask while waiting for the cells to detach. 6. Add 6.0 to 8.0 mL of complete culture medium to the flask and gently pipette up and down to dissociate the cells. Centrifuge at approximately 125 × g for 5 minutes, aspirate the supernatant, gently resuspend the cells in fresh medium, and distribute them into new vessels.
Medium Renewal	Every 2 to 3 days
Subcultivation Ratio	Split optimal, confluent cultures at a standard ratio of 1:6 to 1:10.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	95% Fetal Bovine Serum (FBS) + 5% DMSO (or standard commercial Freeze Medium CM-1)

Cat.No.	Product Name	Price
CSC-RO02609	Cas9 Stable Cell Line - RSC96	Inquiry
CSC-RR00902	GFP Reporter Cell Line - RSC96	Inquiry
CSC-RR00950	Luciferase Reporter Cell Line - RSC96	Inquiry

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* For research use only. Not intended for any clinical use.

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