TC-1 Cell Line

Brief Introduction
Product List

General Information
Organism	Mus musculus, mouse
Cell Line Description	TC-1 is an immortalized and transformed murine epithelial cell line established in 1996 by T.C. Wu and colleagues at the Johns Hopkins University School of Medicine. This cell line was generated by immortalizing primary lung epithelial cells from C57BL/6 mice using the E6 and E7 genes of human papillomavirus type 16 (HPV-16), followed by transformation with the activated human c-Ha-ras (HRAS) oncogene. TC-1 cells grow as an adherent monolayer and exhibit an epithelial-like morphology. In the fields of tumor immunology, virology, and cancer vaccine development, TC-1 is globally recognized as the "gold standard" syngeneic in vitro and in vivo model for studying HPV-associated cancers, such as cervical cancer and HPV-positive head and neck squamous cell carcinoma. Due to their syngeneic nature with C57BL/6 mice, TC-1 cells can be transplanted into immunocompetent mice to evaluate therapeutic HPV vaccines, antigen-specific CD8+ T-cell responses, and immune checkpoint blockade therapies.
Tissue	Lung
Cell Type	Epithelial cell
Strain	C57BL/6
Disease	HPV-Associated Cancer Model / Transformed Lung Epithelial Carcinoma
Morphology	Epithelial-like
Gender	Unspecified
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	2 (BSL-2 is required globally because the cells express integrated HPV-16 E6/E7 viral oncogenes and contain retroviral vectors)
Applications	1. Evaluate "gold-standard" syngeneic models for therapeutic HPV vaccines (e.g., those based on E6/E7 peptides, DNA, or viral vectors); 2. Elucidate the mechanisms underlying the activation of HPV-specific cytotoxic T lymphocytes (CTLs), CD8+ T-cell migration, and immune escape; 3. Conduct preclinical testing of combination immunotherapies involving anti-PD-1/PD-L1, anti-CTLA-4, or STING agonists against HPV-positive tumors; 4. Investigate the synergistic oncogenic mechanisms between high-risk HPV oncoproteins (E6/E7) and the activated Ras signaling pathway; 5. Establish reproducible subcutaneous, pulmonary metastasis, or orthotopic tumor models in immunocompetent syngeneic C57BL/6 mice.
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes, highly tumorigenic and metastatic; forms rapidly growing tumors when injected subcutaneously or intravenously into syngeneic C57BL/6 mice.
Expression Profile	Stably expresses HPV-16 E6 and HPV-16 E7 oncoproteins as well as activated c-Ha-ras.
Oncogenic Mechanism	HPV-16 E6 promotes proteasomal degradation of host p53, while HPV-16 E7 inactivates the retinoblastoma protein (pRb). Synergistic transformation with c-Ha-ras grants full malignant, anchorage-independent growth capabilities.
Growth Kinetics	Rapid adherent expansion; population doubling time is approximately 18 to 24 hours.
Mycoplasma Test	Negative
Culture Conditions and Handling
Subculturing	1. Remove and discard the spent complete culture medium from the culture vessel. 2. Gently rinse the cell monolayer with sterile, Ca2+/Mg2+-free PBS to remove residual serum containing trypsin inhibitors. 3. Add 1.0 to 2.5 mL of 0.25% trypsin-0.53 mM EDTA solution (pre-warmed to the appropriate temperature), ensuring the cell layer is completely covered. 4. Incubate at 37°C for 2 to 5 minutes; monitor closely under an inverted microscope until the cells round up and detach. 5. Add an equal volume of pre-warmed complete growth medium to immediately neutralize trypsin activity. 6. Gently pipette up and down to disperse the cells into a single-cell suspension, centrifuge at approximately 125 × g for 5 minutes, aspirate the supernatant, resuspend the cells in fresh complete medium, and distribute them into new culture vessels.
Thawing Protocol Note	TC-1 cells are sensitive to pH changes during early post-thaw recovery. Prior to introducing the thawed vial contents, place the culture vessel containing fresh complete growth medium into the 5% CO2 incubator for at least 15 minutes to allow the medium to equilibrate to its normal physiological pH range (7.0 to 7.6).
Medium Renewal	2 to 3 times per week
Subcultivation Ratio	Split confluent cultures (70-80% density) at a standard ratio of 1:5 to 1:10. Maintain active growth by splitting before the monolayer reaches 100% confluence.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	90% Complete culture growth medium + 10% DMSO (or 50% RPMI 1640 + 40% FBS + 10% DMSO)

Cat.No.	Product Name	Price
CSC-RR00845	GFP Reporter Cell Line - TC-1	Inquiry
CSC-RR00846	Luciferase Reporter Cell Line - TC-1	Inquiry

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* For research use only. Not intended for any clinical use.

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