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| General Information | |
| Organism | Homo sapiens, human |
| Cell Line Description | M-07e is a human megakaryoblastic leukemia cell line derived from the peripheral blood of a 6-month-old female infant with acute megakaryoblastic leukemia (AMKL). M-07e cells exhibit a lymphoblast-like morphology and grow in suspension culture as single cells or loose clusters. A distinctive feature of this cell line is its strict dependence on cytokines for survival and proliferation, requiring the exogenous addition of interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF). Genetically, the cell line displays complex chromosomal alterations characteristic of pediatric acute megakaryoblastic leukemia. Due to its extreme sensitivity to cytokines, M-07e is widely used as a highly sensitive biological indicator system for assaying the bioactivity of human IL-3 and GM-CSF. Furthermore, it serves as a valuable in vitro model for studying megakaryocytic differentiation pathways, investigating cytokine receptor signal transduction, evaluating tyrosine kinase inhibitors, and conducting functional genomics research on hematopoietic malignancies. |
| Tissue | Peripheral blood / Bone marrow |
| Disease | Acute Megakaryoblastic Leukemia (AMKL); Acute Myeloid Leukemia (AML) |
| Morphology | Lymphoblast-like |
| Gender | Female |
| Age | 6 months |
| Product Format | Frozen |
| Growth Mode | Suspension |
| Biosafety Level | 1 (Biosafety classification is based on U.S. Public Health Service Guidelines) |
| Applications | 1. Quantitative biological assays for human IL-3 and GM-CSF bioactivity; 2. Research on cytokine receptor signaling pathways (JAK/STAT, PI3K/Akt, MAPK); 3. In vitro models of megakaryocyte lineage development and leukemogenesis; 4. Screening and evaluation of targeted kinase inhibitors and anti-leukemia drug candidates. |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Karyotype | Aneuploid |
| Tumorigenic | Yes, in immunocompromised mice under specific cytokine-supplemented conditions |
| Genetic Profile | Cytokine-dependent profile; complex cytogenetic structural variations |
| Expression Markers | Positive for megakaryocytic markers (CD41/GPIIb/IIIa, CD61); CD33 positive; CD34 positive |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Maintain the cell culture by adding fresh medium or replacing the medium. 2. Establish the culture by centrifuging (1,000 rpm, 5 minutes) and resuspending the cells (at a density of 2 × 10⁵ to 4 × 10⁵ viable cells/mL). 3. Ensure that fresh cytokines are added whenever the medium is replaced. 4. Do not allow the cell concentration to exceed 1 × 10⁶ cells/mL. 5. Gently resuspend the cells to create a single-cell suspension before subculturing them into a new culture vessel. |
| Medium Renewal | Every 2 to 3 days |
| Subcultivation Ratio | Maintain cell density between 2 × 10⁵ and 1 × 10⁶ cells/mL (split ratio approximately 1:2 to 1:4) |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | Complete growth medium supplemented with 5% to 10% (v/v) DMSO |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RO0847 | Human PDCD1 Stable Cell Line - M-07e | Inquiry |
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