JHH-7 Cell Line

Brief Introduction
Product List

General Information
Organism	Homo sapiens, human
Cell Line Description	JHH-7 is a human hepatocellular carcinoma (HCC) cell line established by Homma, Fujise, and Nagamori in 1990. It was derived from a 53-year-old Japanese male patient with liver cirrhosis and HBsAg-positive hepatocellular carcinoma. JHH-7 cells exhibit an epithelioid, polygonal morphology and grow as an adherent monolayer. Classified as Edmondson grade III, the cell line retains key characteristics of differentiated hepatocytes, including the synthesis of albumin, alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), and ferritin. Genetically, the cells harbor a p.Arg267Pro point mutation in the TP53 gene, a C228T hotspot mutation in the TERT promoter, and a deletion within the APC-binding domain of the AXIN1 gene. Furthermore, the cells contain only two incomplete, integrated copies of the HBV genome, which are incapable of producing infectious viral particles. JHH-7 serves as an effective model for evaluating p53 activators and inhibitors of the Wnt/β-catenin signaling pathway. It is widely used in research concerning the mechanisms of HBV-associated hepatocarcinogenesis, hepatocyte differentiation, p53 activation, and the response to anticancer drugs in both in vitro and xenograft models.
Tissue	Liver (primary tumor)
Disease	Hepatocellular Carcinoma (HCC); Edmondson grade III
Morphology	Epithelial-like; polygonal
Gender	Male
Age	53 years
Ethnicity	Japanese
Product Format	Frozen
Growth Mode	Adherent
Biosafety Level	1 (Biosafety classification is based on U.S. Public Health Service Guidelines)
Applications	1. Research on HBV integration and the mechanisms underlying HBV-related hepatocarcinogenesis 2. Studies on hepatocyte differentiation and the expression of biomarkers (albumin, AFP, CEA) in hepatocellular carcinoma (HCC) 3. Functional analysis of p53 and investigation of the Wnt/β-catenin signaling pathway in hepatocellular carcinoma 4. Pharmacogenomics and chemosensitivity screening for liver cancer (utilizing databases such as CCLE, GDSC, DepMap, and LIMORE) 5. Establishment of orthotopic and subcutaneous xenograft models in nude mice (including intrahepatic metastasis models) 6. Research on bioartificial liver support systems and hepatic drug metabolism
Shipped In	Dry ice
Storage Temperature	−196°C
Characteristics
Tumorigenic	Yes, forms tumors in KSN nude mice with intrahepatic metastasis
Expression Markers	Synthesizes and secretes albumin, alpha-fetoprotein (AFP), CEA and ferritin
Mycoplasma Test	Negative
Viral Status	HBV genome integrated as two incomplete non-infectious copies; no HBsAg secretion. Negative for EBV, HCV, HTLV-1, HTLV-2, HIV-1, HIV-2, CMV, BKV, JCV, HPV18, HAV and parvovirus B19 by PCR
Culture Conditions and Handling
Subculturing	1. Remove and discard the culture medium. 2. Briefly rinse the cell monolayer with PBS (Ca²⁺/Mg²⁺-free) to thoroughly remove residual serum. 3. Add 2.0 to 3.0 mL of 0.25% Trypsin–0.53 mM EDTA solution, and observe under an inverted microscope until the cell monolayer has dispersed (typically takes 2 to 5 minutes). 4. Add 6.0 to 8.0 mL of complete growth medium, and gently pipette to prepare a single-cell suspension. 5. Aliquot the cell suspension into new culture vessels.
Medium Renewal	1 to 2 times per week
Subcultivation Ratio	Split at a ratio of 1:3 to 1:6 when the monolayer reaches approximately 80% confluence.
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C
Cryopreservation	Complete growth medium (Williams' E) + 20% FBS + 10% DMSO

Cat.No.	Product Name	Price
CSC-RR01048	mScarlet/LUC Reporter Cell Line - JHH-7	Inquiry

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