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| General Information | |
| Organism | Rattus norvegicus, rat |
| Cell Line Description | NRK-52E is a normal rat renal tubular epithelial cell line established in 1978 by Joseph De Larco and George Todaro at the National Cancer Institute (NCI). The cell line was isolated from a clone derived from a mixed-cell culture of Osborne-Mendel rat kidney (NRK). NRK-52E cells grow as an adherent monolayer, exhibiting a characteristic polygonal structure and a cobblestone-like morphology. In the fields of nephrology, renal toxicology, and cell biology, NRK-52E is recognized as the rodent in vitro "gold standard" model for studying renal proximal tubular epithelial function. It serves as a vital platform for assessing nephrotoxicity, investigating acute kidney injury (AKI), exploring transforming growth factor-beta (TGF-β)-induced tubulointerstitial fibrosis, and elucidating the dynamics of epithelial-mesenchymal transition (EMT). |
| Tissue | Kidney; derived from normal renal cortex / tubule |
| Cell Type | Renal Proximal Tubular Epithelial Cell |
| Strain | Osborne-Mendel |
| Disease | Normal / Non-malignant tissue origin |
| Morphology | Epithelial-like; polygonal cells growing as an adherent monolayer sheet |
| Gender | Unspecified |
| Age | Unspecified |
| Product Format | Frozen |
| Growth Mode | Adherent |
| Biosafety Level | 1 (Biosafety classification matches standard laboratory safety parameters for non-infectious, non-malignant rodent primary-like lines) |
| Applications | 1. Utilize rodent in vitro models—the "gold standard"—to evaluate nephrotoxicity and renal tubular cell injury induced by heavy metals (e.g., cadmium, cisplatin), contrast agents, and antibiotics; 2. Elucidate the mechanisms underlying TGF-β1-mediated signaling cascades, extracellular matrix (collagen, fibronectin) accumulation, and tubulointerstitial fibrosis; 3. Establish mechanistic models of epithelial-mesenchymal transition (EMT) in renal tubular cells; 4. Investigate renal tubular transport systems, receptor-mediated endocytosis, and hypoxia-reoxygenation injury; 5. Evaluate the efficacy of protective therapies, anti-fibrotic drugs, and antioxidants against acute kidney injury (AKI). |
| Shipped In | Dry ice |
| Storage Temperature | −196°C |
| Characteristics | |
| Tumorigenic | No, non-tumorigenic under standard physiological conditions; displays contact inhibition and normal epithelial polarity. |
| Transformant | None; unmanipulated, non-malignant clonal line isolated from normal kidney tissue. |
| Epithelial Lineage & Markers | They express classic proximal tubule markers, including E-cadherin, cytokeratins, α-smooth muscle actin (α-SMA; upon TGF-β induction), and functional renal transporters; and they exhibit significant responses to epidermal growth factor (EGF) and transforming growth factor. |
| Fibrotic & EMT Responsiveness | It is highly responsive to exogenous TGF-β1. Treatment induces a significant and quantifiable phenotypic transition, resulting in a myofibroblast-like morphology accompanied by elevated expression levels of type I collagen, type IV collagen, fibronectin, and PAI-1. |
| Growth Kinetics | Rapid adherent expansion; typical population doubling time spans approximately 18 to 24 hours. |
| Mycoplasma Test | Negative |
| Culture Conditions and Handling | |
| Subculturing | 1. Completely aspirate and discard the complete culture medium from the culture vessel. 2. Gently rinse the cell monolayer with sterile PBS (without Ca2+/Mg2+) to remove residual serum containing active trypsin inhibitors. 3. Add 1.0 to 2.5 mL of 0.25% trypsin-0.53 mM (0.02%) EDTA solution, pre-warmed to the appropriate temperature, ensuring the cell layer is completely covered. 4. Incubate at 37°C for 2 to 5 minutes; monitor under an inverted microscope until the cells become rounded, loosen, and completely detach. 5. Add an equal volume of complete growth medium, pre-warmed to the appropriate temperature, to immediately neutralize trypsin activity. 6. Gently pipette the cell suspension to disperse the cells, centrifuge at approximately 125 × g for 5 minutes, carefully discard the supernatant, gently resuspend the cells in fresh complete growth medium, and distribute them into new culture vessels. |
| Thawing Protocol Note | NRK-52E cells are sensitive to pH changes during early post-thaw recovery. Prior to introducing thawed vial contents, place the culture vessel containing fresh complete growth medium into the 5% CO2 incubator for at least 15 minutes to allow the medium to equilibrate to its physiological pH range (7.0 to 7.6). |
| Medium Renewal | 2 to 3 times per week |
| Subcultivation Ratio | Split confluent cultures (80-90% density) at a standard ratio of 1:3 to 1:8. Maintain active growth by subculturing before cells become completely overgrown or piled up. |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5%; Temperature: 37°C |
| Cryopreservation | 90% Complete culture growth medium + 10% DMSO (or 50% DMEM + 40% Serum + 10% DMSO) |
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR00814 | Luciferase Reporter Cell Line - NRK-52E | Inquiry |
| CSC-RR00815 | GFP Reporter Cell Line - NRK-52E | Inquiry |
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