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Protocols

protocols
Protocol

Transformation of drug-resistant plasmid DNA Protocol

Experiment Tags: Plasmid DNA, Transformation, Drug-Resistant Plasmids

Experiment Summary: This article provides a detailed protocol for the assay with hands-on tips for the LnRNA pulldown assay, which is commonly used to identify protein partners of the cellular environment that interact with InRNA.

protocols
Protocol

CytoTrap Two-Hybrid Screening Assay Protocol

Experiment Tags: Cytotrap System, Yeast Two-Hybrid Screen, Protein Interaction

Experiment Summary: In this protocol, we introduce a method to study RNA-protein interactions, which will facilitate researchers to carry out circRNA related experiments.

protocols
Protocol

In Vivo Analysis of Neutrophil Infiltration during LPS-induced Peritonitis Protocol

Experiment Tags: Inflammation, Peritonitis, Sepsis, In Vivo Imaging, Myeloperoxidase

Experiment Summary: In this protocol, we describe a method for dsRNA isolation. Using this method, we can readily isolate viral dsRNA from a small amount of plant material, and can process numerous samples simultaneously.

protocols
Protocol

Design and Production Protocols of Plasmids for Base-Editing Experiments

Experiment Tags: Base-Editing, CRISPR-Cas9

Experiment Summary: In this protocol, we describe in detail the necessary steps to deliver mRNA-LNP to the murine liver and, following intravenous injection of eGFP mRNA-LNP, verify transfection efficiency using flow cytometry and liver cell specificity using immunofluorescence analyses

protocols
Protocol

LncRNA Pulldown Combined with Mass Spectrometry to Identify the Novel lncRNA-Associated Proteins Protocol

Experiment Tags: Long Noncoding RNAs, RNA-Protein Interaction, Mass Spectrometry

Experiment Summary: In this protocol, we demonstrate a detailed protocol for AAV-mediated intra-embryo knock-in in laboratory mice. This technique does not require either micromanipulation or germline competent pluripotent stem cells.