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Protocols

protocols
Protocol

Generation of Stable Cell Lines Using Lentivirus Protocol

Experiment Tags: Cell, Cell Line Generation, Lentivirus

Experiment Summary: This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

protocols
Protocol

Determining Antibiotic Killing Curve for Stable Cell Line Generation Protocol

Experiment Tags: Cell, Cell Line Generation

Experiment Summary: Since mammalian cells sensitivity to antibiotics varies from one cell type to another, it is essential to determine the minimum concentration of antibiotic required to kill non-transfected or non-transduced cells by separate kill curve experiments.

protocols
Protocol

Converting Adherent Cells to Suspension Cells (Serum Dilution) Protocol

Experiment Tags: Cell, Adherent Cells, Suspension Cells

Experiment Summary: If the cell viability is >85% and the generation time is similar to that observed with the original medium, it suggests that the cells adapt to growth in current condition.

protocols
Protocol

Assay of Cell Apoptosis (Annexin-V-FITC)

Experiment Tags: Detection, Apoptosis, Annexin-V-FITC, Cells

Experiment Summary: This experiment introduces the principle and procedure of the assay of cell apoptosis (Annexin-V-FITC).

protocols
Protocol

Generation of Luciferase-Expressing Tumor Cell Lines Protocol

Experiment Tags: Lentivirus, Tumor, Lymphoma, Luciferase, GFP, Mouse

Experiment Summary: This protocol describes the construction of a glycine oxidase mutant library by random mutagenesis and the use of a phage T7 lysis-based approach for high-throughput screening of oxidase activity, among other things.