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Protocols

protocols
Protocol

Determining Antibiotic Killing Curve for Stable Cell Line Generation Protocol

Experiment Tags: Cell, Cell Line Generation

Experiment Summary: Since mammalian cells sensitivity to antibiotics varies from one cell type to another, it is essential to determine the minimum concentration of antibiotic required to kill non-transfected or non-transduced cells by separate kill curve experiments.

protocols
Protocol

Adenovirus Construction by Homologous Recombination in Bacteria Protocol

Experiment Tags: Adenovirus

Experiment Summary: In our protocol, the recombination between the shuttle plasmid and the adenovirus genome contained in the backbone plasmid is performed in the E. coli strain. Positive recombinants are selected by resistance to an antibiotic.

protocols
Protocol

Generation of Stable Cell Lines Using Lentivirus Protocol

Experiment Tags: Cell, Cell Line Generation, Lentivirus

Experiment Summary: This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

protocols
Protocol

Generation and Amplification of Recombinant Adenovirus Protocol

Experiment Tags: Adenovirus, Recombinant Adenovirus

Experiment Summary: Conventional approaches to producing small volumes of viral vectors involve culturing cells in stationary and adherent cultures.

protocols
Protocol

LPS Extraction Protocol

Experiment Tags: Lipopolysaccharide, LPS Extraction

Experiment Summary: Here, we describe a hot aqueous-phenol method for the isolation and purification of LPS from gram-negative bacterial cells.

protocols
Protocol

BCA Library Construction Protocol

Experiment Tags: BCA Library, Library, Library Construction

Experiment Summary: The size-fractionated DNA is cloned into a BAC vector and transformed into E. coli by electrical shock. The transformants are arrayed into microtiter dishes and high-density replica filters are prepared to facilitate screening of the library.

protocols
Protocol

cDNA Library Construction Protocol

Experiment Tags: cDNA Library, Library, Library Construction

Experiment Summary: To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA).

protocols
Protocol

Viral Titering-TCID50 Assay Protocol

Experiment Tags: Viral Titering, TCID50 Assay

Experiment Summary: A TCID50 was found of greatest value when lack of experimental material or costs of analysis precludes extensive replicate titer determinations but good estimates of titers and treatment differences are essential.

protocols
Protocol

Viral Titering-Plaque Assay Protocol

Experiment Tags: Viral Titering, Plaque Assay

Experiment Summary: After fixing and staining the infected cellular monolayer, plaques are counted so that titer viral stock samples in terms of plaque forming units (pfu) per milliliter.

protocols
Protocol

Transient Transfection Protocol

Experiment Tags: Cell, Transfection, Transient transfection

Experiment Summary: Transient transfection means that the constructed plasmids are introduced into mammalian cells in some ways, but the foreign genes of the plasmid are not integrated into the genome of the mammalian cell.

* For research use only. Not intended for any clinical use.
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