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Experiment Tags: RSV Transcription, Minigenome, Polymerase, Luciferase Assay, Real-Time Quantitative PCR Assay
Experiment Summary: This protocol describes the coupling of (i) “live” in vitro RNA transcription with (ii) binding by a radiolabeled, pre-formed tRNA followed by native gel electrophoresis and phosphorimager scan to visualize the complex.
Experiment Tags: CircRNAs, RNA Pull-Down, RNA-Protein Interactions
Experiment Summary: In this protocol, we report a protocol in infected mice with different virus doses and strains to explore how an inhibitor of lysine-specific demethylase (LSD1) impacts disease progression.
Experiment Tags: Diagnosis, RNA Virus, Replicative Intermediate, dsRNA Isolation, dsRNA-Binding Protein
Experiment Summary: This protocol describes the design of divergent primers which face away from each other on the linear RNA, so that they can only amplify the circRNAs, and not the linear RNAs with the same sequence.
Experiment Tags: CRISPR-Cas9, Off-Target Activity, Chromosomal Translocation, LAM-HTGTS, iHTGTS
Experiment Summary: This protocol describes the use of the 3rd generation lentiviral system which uses three different plasmids for generating stable cell lines.
Experiment Tags: Nucleoside-Modified mRNA, Lipid Nanoparticle, Liver Regeneration, Protein Expression, Retro-Orbital Injection
Experiment Summary: This protocol describes the entire process of transient transformation of maize embryos using particle bombardment.
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