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Protocols

protocols
Protocol

T7E1 Assay Protocol

Experiment Tags: T7E1, T7E1 Assay

Experiment Summary: Direct PCR product could be used for T7E1 detection.

protocols
Protocol

Standard PCR Protocol

Experiment Tags: PCR, Standard PCR

Experiment Summary: Taq polymerase is frequently used in the polymerase chain reaction (PCR), for it is able to withstand repeated heating to 95 °C without significant loss of activity, an efficient method for greatly amplifying the quantity of DNA templates.

protocols
Protocol

RT-PCR Protocol

Experiment Tags: RT-PCR, PCR

Experiment Summary: RNA is first reverse transcribed into cDNA using a reverse transcriptase as described here, the resulting cDNA is used as templates for subsequent PCR amplification using primers specific for one or more genes.

protocols
Protocol

RNAi Protocol

Experiment Tags: RNAi

Experiment Summary: RNAi consists of inititation and effector steps. At inititation steps, the dsRNAs get processed into 21-23 nucleotide (nt) small interfering RNAs (siRNAs) by an RNase III-like enzyme called Dicer.

protocols
Protocol

Lentivirus Packaging Protocol

Experiment Tags: Lentivirus packaging protocol, lentivirus packaging, lentivirus, lentivirus production

Experiment Summary: Lentiviral packaging is a key method in molecular biology for delivering genetic material to target cells. By utilizing recombinant lentiviral particles, this protocol allows for highly efficient gene transfer, which is critical for research and therapeutic applications. This review discusses detailed lentiviral packaging protocols and highlights best practices for achieving optimal viral titers.

protocols
Protocol

PERT Assay Protocol

Experiment Tags: PERT Assay, Product-enhanced RT

Experiment Summary: Sensitivity was greatly increased when a PCR amplification step of the synthesized cDNA was introduced prior to product detection. These types of assays are known as product-enhanced RT (PERT) assays.

protocols
Protocol

Limiting Dilution Protocol

Experiment Tags: Dilution, Cell

Experiment Summary: Limiting dilution analysis attempts to determine the frequency of cells having a particular function that are present in a mixed population of cells. So limiting dilution analysis is an easy-operated method that used to measure the abundance of cells able to perform a particular function.

protocols
Protocol

Lentivirus Transduction Protocol

Experiment Tags: Lentivirus, Lentivirus Transduction

Experiment Summary: Lentivirus is a modified HIV virus that must be handled with caution although it cannot replicate in a host.

protocols
Protocol

Designing sgRNA using CRISPR Protocol

Experiment Tags: sgRNA, CRISPR

Experiment Summary: Selection of sgRNAs for an experiment needs to balance two aspects: maximizing on-target activity while minimizing off-target activity, which sounds obviously but can often require thorough decisions.

protocols
Protocol

Gene Overexpression in Cell Lines Protocol

Experiment Tags: Cell, Cell Line

Experiment Summary: The fundamental principle is to add regulatory elements to the upstream of the target gene through artificial construction, so that genes can be transcribed and translated efficiently under controlled conditions.

* For research use only. Not intended for any clinical use.
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