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Protocols

protocols
Protocol

Cell Immortalization Protocol Using Lentiviral Particles

Experiment Tags: Cell Immortalization, Lentiviral Transduction

Experiment Summary: The protocol introduces the definition of cell immortalization and commonly used viral transfection methods, and describes in detail the steps to generate immortalized cells using prefabricated cell immortalization lentiviral particles.

protocols
Protocol

Rapid Modular CAR-T Generation with CRISPR/Cpf1 and AAV Systems Protocol

Experiment Tags: CRISPR, Cpf1, AAV, CAR-T, T Cell, Cancer, Immunotherapy

Experiment Summary: This protocol describes a new method, the AAV-Cpf1 KIKO system, using a combination of viral and non-viral approaches to generate a stable CAR-T with homology-directed repair (HDR) knock-in and immune checkpoint knockout at high efficiency in one step.

protocols
Protocol

Lentivirus-mediated Conditional Gene Expression Protocol

Experiment Tags: NLR, NOD1, Lentivirus, Stable Expression, TRE, Tetracycline

Experiment Summary: In this protocol, we describe a method for stable, conditional expression of Nod-Like receptors (NLRs) in THP-1 cells using a lentiviral expression system.

protocols
Protocol

Generation of Luciferase-Expressing Tumor Cell Lines Protocol

Experiment Tags: Lentivirus, Tumor, Lymphoma, Luciferase, GFP, Mouse

Experiment Summary: In this protocol, we describe methods for the production of stable luciferase expressing tumor cell lines by lentiviral transduction.

protocols
Protocol

Transformation of drug-resistant plasmid DNA Protocol

Experiment Tags: Plasmid DNA, Transformation, Drug-Resistant Plasmids

Experiment Summary: This experiment describes the method of transferring plasmid DNA molecules into E. coli and how to screen cell clones with plasmid DNA, i.e. transformants.

protocols
Protocol

Preparation of Genomic Overexpression Library Protocol

Experiment Tags: Genomic, Overexpression, Library

Experiment Summary: This protocol might work for searching complementation of other gene deletion mutants that have a good selective phenotype.

protocols
Protocol

Maize Embryo Transient Transformation by Particle Bombardment Protocol

Experiment Tags: Particle bombardment, Maize embryos, Promoter expression, Quantitative analysis

Experiment Summary: This protocol describes the entire process of transient transformation of maize embryos using particle bombardment.

protocols
Protocol

Ex vivo Culture and Lentiviral Transduction Protocol of Benign Prostatic Hyperplasia (BPH) Samples

Experiment Tags: Ex vivo tissue culture model, Lentiviral, Proliferation, Oncogenic , Transformation, Prostate

Experiment Summary: This protocol describes how to generate lentiviral vectors and particules, successfully transduce human prostate tissues, induce exogenous gene expression, and assess cellular proliferation.

protocols
Protocol

Protocol for Generating Stably Expressed Mammalian Cell Lines Using Lentivirus

Experiment Tags: Lentivirus, Stable cell lines, Gene knockdown, Gene over-expression, Mammalian cell lines, Transduction

Experiment Summary: This protocol describes the use of the 3rd generation lentiviral system which uses three different plasmids for generating stable cell lines.

protocols
Protocol

Protocol for Detection and Analysis of Cyclic RNA Using RT-PCR

Experiment Tags: Circular RNA, Backsplice junction, Divergent primer, RT-PCR (RT-PCR), RNase R (RNase R)

Experiment Summary: This protocol describes the design of divergent primers which face away from each other on the linear RNA, so that they can only amplify the circRNAs, and not the linear RNAs with the same sequence.

* For research use only. Not intended for any clinical use.
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