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Experiment Tags: Plasmid purification, Plasmid purification
Experiment Summary: Plasmids are small circular deoxyribonucleic acids (DNA) outside the chromosomes that replicate independently of chromosomal DNA. Although budding yeast and fission yeast can retain plasmids, the host of plasmids is almost always bacteria. This small circular DNA is widely used as a DNA vector in the fields of molecular biology, biochemistry, biotechnology, cell biology, etc.
Experiment Tags: E. coli Expression, Cloning, plasmid preparation
Experiment Summary: Recombinant protein expression in Escherichia coli (E. coli) is widely used in molecular biology and biotechnology due to its ease, cost-effectiveness, and rapid growth. This E. coli expression protocol outlines a standardized workflow for cloning, transformation, protein expression, and purification in the E. coli system, ensuring reproducibility and efficiency.
Experiment Tags: Dilution, Cell
Experiment Summary: Limiting dilution analysis attempts to determine the frequency of cells having a particular function that are present in a mixed population of cells. So limiting dilution analysis is an easy-operated method that used to measure the abundance of cells able to perform a particular function.
Experiment Tags: Cell, Cell Line
Experiment Summary: The fundamental principle is to add regulatory elements to the upstream of the target gene through artificial construction, so that genes can be transcribed and translated efficiently under controlled conditions.
Experiment Tags: Cell, Transfection, Transient transfection
Experiment Summary: Transient transfection means that the constructed plasmids are introduced into mammalian cells in some ways, but the foreign genes of the plasmid are not integrated into the genome of the mammalian cell.
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