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Molecular Biology

protocols
Protocol

CRISPR Plasmid Transfection Protocol

Experiment Tags: CRISPR, CRISPR Plasmid

Experiment Summary: The CRISPR/Cas9 system has two critical parts: sgRNA sequence and Cas9 sequence. These two sequences could be cloned into separated plasmids or combined into one plasmid, each format has its own set of advantages and shares a similar delivery procedure.

protocols
Protocol

Codon Optimization Protocol

Experiment Tags: Codon Optimization, Codon

Experiment Summary: Codon optimization is one of the key steps in achieving the high level expression of target gene. There are some key factors for consideration including transcription efficiency, translation efficiency, gene synthesis and protein folding.

* For research use only. Not intended for any clinical use.
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