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Molecular Biology

protocols
Protocol

CytoTrap Two-Hybrid Screening Assay Protocol

Experiment Tags: Cytotrap System, Yeast Two-Hybrid Screen, Protein Interaction

Experiment Summary: This protocol describes the construction of a cDNA library from the model plant Arabidopsis and a procedure for screening interaction proteins of AtSR1/CAMTA3, a Ca2+/CaM-regulated transcription factor from this library.

protocols
Protocol

Transformation of drug-resistant plasmid DNA Protocol

Experiment Tags: Plasmid DNA, Transformation, Drug-Resistant Plasmids

Experiment Summary: This experiment describes the method of transferring plasmid DNA molecules into E. coli and how to screen cell clones with plasmid DNA, i.e. transformants.

protocols
Protocol

Protocol for Detection and Analysis of Cyclic RNA Using RT-PCR

Experiment Tags: Circular RNA, Backsplice junction, Divergent primer, RT-PCR (RT-PCR), RNase R (RNase R)

Experiment Summary: This protocol describes the design of divergent primers which face away from each other on the linear RNA, so that they can only amplify the circRNAs, and not the linear RNAs with the same sequence.

protocols
Protocol

Construction of Transgenic Zebrafish Protocol

Experiment Tags: Green Fluorescent Protein, Zebrafish, Transgenic

Experiment Summary: In this experiment, EGFP-containing plasmid was introduced into zebrafish to observe its expression in animals. In zebrafish, green fluorescent protein could be observed under fluorescence microscope from proto-intestinal embryo to emergence stage.

protocols
Protocol

Transmembrane of RNA Samples (Siphon Blotting Method)

Experiment Tags: Transmembrane, Siphon Blotting, RNA Samples

Experiment Summary: This experiment introduces the procedure of transmembrane (i.e., siphon blotting) of RNA samples, etc.

protocols
Protocol

LPS Extraction Protocol

Experiment Tags: Lipopolysaccharide, LPS Extraction

Experiment Summary: Here, we describe a hot aqueous-phenol method for the isolation and purification of LPS from gram-negative bacterial cells.

protocols
Protocol

T7E1 Assay Protocol

Experiment Tags: T7E1, T7E1 Assay

Experiment Summary: Direct PCR product could be used for T7E1 detection.

protocols
Protocol

RNAi Protocol

Experiment Tags: RNAi

Experiment Summary: RNAi consists of inititation and effector steps. At inititation steps, the dsRNAs get processed into 21-23 nucleotide (nt) small interfering RNAs (siRNAs) by an RNase III-like enzyme called Dicer.

protocols
Protocol

PERT Assay Protocol

Experiment Tags: PERT Assay, Product-enhanced RT

Experiment Summary: Sensitivity was greatly increased when a PCR amplification step of the synthesized cDNA was introduced prior to product detection. These types of assays are known as product-enhanced RT (PERT) assays.

protocols
Protocol

Designing sgRNA using CRISPR Protocol

Experiment Tags: sgRNA, CRISPR

Experiment Summary: Selection of sgRNAs for an experiment needs to balance two aspects: maximizing on-target activity while minimizing off-target activity, which sounds obviously but can often require thorough decisions.

* For research use only. Not intended for any clinical use.
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