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Molecular Biology

protocols
Protocol

PERT Assay Protocol

Experiment Tags: PERT Assay, Product-enhanced RT

Experiment Summary: Sensitivity was greatly increased when a PCR amplification step of the synthesized cDNA was introduced prior to product detection. These types of assays are known as product-enhanced RT (PERT) assays.

protocols
Protocol

LPS Extraction Protocol

Experiment Tags: Lipopolysaccharide, LPS Extraction

Experiment Summary: Here, we describe a hot aqueous-phenol method for the isolation and purification of LPS from gram-negative bacterial cells.

protocols
Protocol

Transmembrane of RNA Samples (Siphon Blotting Method)

Experiment Tags: Transmembrane, Siphon Blotting, RNA Samples

Experiment Summary: This experiment introduces the procedure of transmembrane (i.e., siphon blotting) of RNA samples, etc.

protocols
Protocol

Construction of Transgenic Zebrafish Protocol

Experiment Tags: Green Fluorescent Protein, Zebrafish, Transgenic

Experiment Summary: In this experiment, EGFP-containing plasmid was introduced into zebrafish to observe its expression in animals. In zebrafish, green fluorescent protein could be observed under fluorescence microscope from proto-intestinal embryo to emergence stage.

protocols
Protocol

Protocol for Detection and Analysis of Cyclic RNA Using RT-PCR

Experiment Tags: Circular RNA, Backsplice junction, Divergent primer, RT-PCR (RT-PCR), RNase R (RNase R)

Experiment Summary: This protocol allows studying of inflammatory response induced by LPS that affect neutrophil infiltration by tracking myeloperoxidase (MPO) activity in vivo.