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Cell Biology

protocols
Protocol

Optimized Protocol to Create Deletion in Adherent Cell Lines Using CRISPRCas9 System

Experiment Tags: Single Cell, Genomics, Molecular Biology, Gene Expression, CRISPR

Experiment Summary: This protocol describes the detailed steps for generating knockout cell lines (coding or non-coding regions) using CRISPR/Cas9 tools.

protocols
Protocol

Generation of Luciferase-Expressing Tumor Cell Lines Protocol

Experiment Tags: Lentivirus, Tumor, Lymphoma, Luciferase, GFP, Mouse

Experiment Summary: In this protocol, we describe methods for the production of stable luciferase expressing tumor cell lines by lentiviral transduction.

protocols
Protocol

Assay of Cell Apoptosis (Annexin-V-FITC)

Experiment Tags: Detection, Apoptosis, Annexin-V-FITC, Cells

Experiment Summary: This experiment introduces the principle and procedure of the assay of cell apoptosis (Annexin-V-FITC).

protocols
Protocol

Converting Adherent Cells to Suspension Cells (Serum Dilution) Protocol

Experiment Tags: Cell, Adherent Cells, Suspension Cells

Experiment Summary: If the cell viability is >85% and the generation time is similar to that observed with the original medium, it suggests that the cells adapt to growth in current condition.

protocols
Protocol

Determining Antibiotic Killing Curve for Stable Cell Line Generation Protocol

Experiment Tags: Cell, Cell Line Generation

Experiment Summary: Since mammalian cells sensitivity to antibiotics varies from one cell type to another, it is essential to determine the minimum concentration of antibiotic required to kill non-transfected or non-transduced cells by separate kill curve experiments.

protocols
Protocol

Generation of Stable Cell Lines Using Lentivirus Protocol

Experiment Tags: Cell, Cell Line Generation, Lentivirus

Experiment Summary: This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

protocols
Protocol

Transient Transfection Protocol

Experiment Tags: Cell, Transfection, Transient transfection

Experiment Summary: Transient transfection means that the constructed plasmids are introduced into mammalian cells in some ways, but the foreign genes of the plasmid are not integrated into the genome of the mammalian cell.

protocols
Protocol

Limiting Dilution Protocol

Experiment Tags: Dilution, Cell

Experiment Summary: Limiting dilution analysis attempts to determine the frequency of cells having a particular function that are present in a mixed population of cells. So limiting dilution analysis is an easy-operated method that used to measure the abundance of cells able to perform a particular function.

protocols
Protocol

Gene Overexpression in Cell Lines Protocol

Experiment Tags: Cell, Cell Line

Experiment Summary: The fundamental principle is to add regulatory elements to the upstream of the target gene through artificial construction, so that genes can be transcribed and translated efficiently under controlled conditions.

* For research use only. Not intended for any clinical use.
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