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Cell Biology

protocols
Protocol

Converting Adherent Cells to Suspension Cells (Serum Dilution) Protocol

Experiment Tags: Cell, Adherent Cells, Suspension Cells

Experiment Summary: If the cell viability is >85% and the generation time is similar to that observed with the original medium, it suggests that the cells adapt to growth in current condition.

protocols
Protocol

Assay of Cell Apoptosis (Annexin-V-FITC)

Experiment Tags: Detection, Apoptosis, Annexin-V-FITC, Cells

Experiment Summary: This experiment introduces the principle and procedure of the assay of cell apoptosis (Annexin-V-FITC).

protocols
Protocol

Generation of Luciferase-Expressing Tumor Cell Lines Protocol

Experiment Tags: Lentivirus, Tumor, Lymphoma, Luciferase, GFP, Mouse

Experiment Summary: This protocol describes the construction of a glycine oxidase mutant library by random mutagenesis and the use of a phage T7 lysis-based approach for high-throughput screening of oxidase activity, among other things.

protocols
Protocol

Optimized Protocol to Create Deletion in Adherent Cell Lines Using CRISPR/Cas9 System

Experiment Tags: Single Cell, Genomics, Molecular Biology, Gene Expression, CRISPR

Experiment Summary: In this article, we described an improved HTGTS (iHTGTS) method, in which size-selection beads were used to enhance reaction efficiency and a new primer system was designed to be compatible with Illumina Hiseq sequencing. iHTGTS is a powerful method for comprehensively assessing Cas9 off-target effect.

protocols
Protocol

Protocol for Rate-Controlled Cryopreservation and Thawing of Mammalian Cells

Experiment Tags: Biological Techniques, Cryopreservation, Cooling, Freezing

Experiment Summary: In this protocol, we describe the general procedure for cryopreservation of cultured cells.