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Experiment Tags: Cell Culture
Experiment Summary: Cell culture is a fundamental technology in biological research, but it often faces challenges that may affect experimental results. The following will analyze common problems in cell culture and provide practical solutions.
Experiment Tags: Cell Transfection, Liposome Transfection
Experiment Summary: For mammalian cell transfection and protein expression, choosing the right transfection method and transfection reagent plays a decisive role in the success rate of transfection. This article mainly lists some methods, principles and applicability of transient transfection and stable transfection, and lists the steps and precautions of liposome cell transfection.
Experiment Tags: Mammalian cell, cell subculture
Experiment Summary: The basic principles, experimental steps and precautions for subculture of HEK293 cells and CHO cells will be introduced.
Experiment Tags: Knock-in Cell Lines, Knock-in
Experiment Summary: Because the success of knock-in cell line construction is influenced by various factors, including the length of the knock-in gene fragment, homologous recombination efficiency, and cell line type, which can impact editing efficiency, this also makes the knock-in technology challenging. This article introduces the principles of constructing KI cell lines and the details that need to be paid attention to.
Experiment Tags: Mitochondrial membrane potential, ΔΨm, JC-1 assay, apoptosis, mitochondrial dysfunction, flow cytometry, fluorescence microscopy, cell biology protocol
Experiment Summary: This protocol describes a JC-1–based method for assessing mitochondrial membrane potential (ΔΨm) in cultured cells. By comparing red and green fluorescence signals generated by JC-1 aggregates and monomers, respectively, changes in ΔΨm can be detected as an early indicator of mitochondrial dysfunction and apoptosis. The procedure applies to both flow cytometry and fluorescence microscopy and includes detailed guidance on cell preparation, staining, data acquisition, and analysis.
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