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Cat.No. : CSC-RR0546 Host Cell: OVCAR8
| Cat. No. | CSC-RR0546 |
| Description | This cell line is engineered to stably overexpress luciferase reporter gene in OVCAR8. |
| Gene | Luciferase |
| Host Cell | OVCAR8 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Proliferation And Apoptosis Adaptor (PEA15) is a 15kDa multifunctional phosphoprotein that plays crucial roles in regulating cancer cell proliferation and apoptosis. Researchers analyzed PEA15 expression in ovarian cancer tissues, finding it significantly upregulated compared to normal tissues. High PEA15 expression correlated with advanced cancer stages and poor prognosis. Knockdown inhibited cell proliferation and induced apoptosis, while overexpression promoted proliferation. miR-212 negatively regulated PEA15 expression and affected cell proliferation and apoptosis. PEA15 emerges as a promising biomarker and therapeutic target in ovarian cancer treatment.
Figure 1. Researchers analyzed miR212 regulation in ovarian cancer (OC) cells. They identified miR212 as a potential PEA15 target via luciferase reporter assays in OVCAR8 cells. MiR212 downregulation in OC tissues correlated with decreased PEA-15 expression. Overexpression of miR212 reduced PEA-15 protein levels, impacting cell proliferation and apoptosis. (Luo Y, et al., 2020)
A: The stability and reliability of luciferase expression in the OVCAR8 Luciferase Reporter Cell Line were ensured through stable transfection techniques, followed by clonal selection to isolate cell lines with consistent luciferase expression levels. Regular monitoring using luciferase assays confirmed sustained expression over time.
A: The OVCAR8 Luciferase Reporter Cell Line serves as a valuable tool for studying cancer biology, including tumor growth, metastasis, and drug responses. Researchers can use this cell line to investigate the efficacy of anticancer compounds or to monitor the activation of specific signaling pathways involved in cancer progression.
A: The OVCAR8 Luciferase Reporter Cell Line is ideal for high-throughput screening assays due to its stable luciferase expression and consistent response to stimuli. Researchers can use this cell line to perform rapid and reliable screening of compounds or treatments for their effects on cellular processes relevant to cancer biology.
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Consistent performance! The Luciferase Reporter Cell Line consistently provides robust signals, ensuring reproducible results and accurate interpretation of experimental outcomes. From investigating oncogenic pathways to screening drug candidates, this cell line serves as a trusted resource for elucidating ovarian cancer biology and developing targeted therapies.
French
05/29/2020
Streamlined experimentation! Its reliable luciferase expression simplifies experimental workflows, facilitating efficient data collection and analysis in ovarian cancer research.
United Kingdom
10/08/2022
The Luciferase Reporter Cell Line in OVCAR8 cells offers sensitive detection of gene expression and pathway activity, providing valuable data for ovarian cancer research. With luciferase bioluminescence, this cell line enables precise monitoring of cellular processes and responses to stimuli, advancing our understanding of ovarian cancer biology.
Germany
01/06/2021
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