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Luciferase Reporter Cell Line-K562

Luciferase Reporter Cell Line-K562

Cat.No. :  CSC-RR0321 Host Cell:  K562

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Cat. No. CSC-RR0321
Description K562-Luciferase cell line stably overexpresses luciferase as a reporter of K562 cells. It is a good ready-to-use cell model for tracing K562 cells.
Gene Luciferase
Host Cell K562
Host Cell Species Homo sapiens (Human)
Stability Validated for at least 10 passages
Application

1. Gene expression studies

2. Protein localization

3. Drug screening and toxicology

4. Live cell imaging

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Source K562
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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The Luc reporter system has several advantages in molecular biology research. Firstly, the light emitted by luciferase is easily detectable and quantifiable, providing a sensitive and reliable measure of gene expression. Secondly, the system is versatile and can be used in various cellular and tissue cultures, as well as in animal models. Thirdly, the Luc reporter can be used to study a wide range of biological processes, including transcription factors, signaling pathways, and gene regulation networks. K562 Cell Line is a human chronic myeloid leukaemia (CML) cell line that has been used extensively in leukaemia research. The cell line was established from a CML patient and has become an important model for studying the pathogenesis and treatment of the disease. Some of the major advantages of using K562 cells include rapid growth, ease of culture, and karyotypic stability. These cells also exhibit a high degree of tumourigenicity, making them suitable for preclinical studies of cancer treatments. In addition, K562 cells express a wide range of cell surface antigens, making them suitable for immune-based studies. In conclusion, the cell line K562 is an important tool for understanding and treating chronic myeloid leukaemia.
The luciferase reporter K562 Cell Line is an important tool for gene expression studies and is widely used in molecular biology and biomedical research. This cell line is derived from the human chronic myeloid leukaemia cell line K562 and has been genetically modified to express the luciferase gene under the control of the relevant promoter. This allows researchers to measure bioluminescence using a luminometer to monitor and quantify the activity of specific promoters or regulatory elements in real time. One of the main advantages of using the luciferase reporter K562 Cell Line - is its high sensitivity and specificity, enabling researchers to detect subtle changes in gene expression. This cell is easy to culture and grows relatively quickly, providing researchers with a stable and reliable experimental system. In addition, this cell line has been shown to have a stable karyotype, ensuring consistent experimental results. In addition, the luciferase reporter K562 Cell Line can be used for a variety of applications, such as studying the effects of different drugs or chemicals on gene expression, investigating the function of specific transcription factors, or studying the regulatory mechanisms of various biological processes. Its versatility and ease of use make it an indispensable tool for researchers in the field of molecular biology and a powerful resource to advance our understanding of gene expression and regulation.
Customer Q&As
How is K562 Cell Line used in the study of CML treatment strategies?

A: K562 Cell Line is used to test the efficacy of different treatment approaches for CML, including tyrosine kinase inhibitors like imatinib, which target the BCR-ABL1 fusion protein, and to investigate resistance mechanisms to these therapies.

What functions does the BCR-ABL1 fusion protein perform in Cell Line-K562?

A: The BCR-ABL1 fusion protein in K562 Cell Line has increased tyrosine kinase activity, which activates multiple signaling pathways that contribute to cell proliferation, survival, and migration, characteristic of CML pathology.

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Customer Reviews
High Luciferase Activity

Luciferase Reporter Cell Line - K562 has strong luciferase activity, providing reliable results for my research.

Canada

03/16/2022

Safe and Efficient

The cell line is safe to use and performs efficiently, providing accurate data for my experiments.

French

03/26/2020

Easy to Cultivate

The luciferase reporter cell line-K562 is easy to culture and handle, saving time and effort for my research process.

Germany

06/25/2022

Affordable

Cost-effective The product is value for money and provides reliable results at a reasonable price.

Canada

05/14/2023

Suitable for a wide range of research

The Luciferase Reporter Cell Line-K562 can be applied to a wide range of research areas and is a useful tool for me to carry out my experiments.

Germany

11/29/2023

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