TCF/LEF-Luc Reporter Cell Line-HEK293T

Name: TCF/LEF-Luc Reporter Cell Line-HEK293T
SKU: CSC-RR0222
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0222

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RR0222
Description	TCF/LEF-Luc Reporter Cell Line-HEK293T stably expresses a secreted luciferase reporter gene under the control of CMV promoter fused to TCF/LEF transcriptional response elements. This cell line is an ideal cellular model for studying the Wnt signaling path
Introduction	TCF/LEF-Luc Reporter Cell Line-HEK293T stably expresses a secreted luciferase reporter gene under the control of CMV promoter fused to TCF/LEF transcriptional response elements. This cell line is an ideal cellular model for studying the Wnt signaling pathway, by monitoring the activity of secreted luciferase. Levels of secreted luciferase can be easily detected in the supernatant.
Target Gene	Luciferase
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Source	HEK293T
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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