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Cat.No. : CSC-RR0551 Host Cell: NCI-H82
| Cat. No. | CSC-RR0551 |
| Description | This cell line is engineered to stably express luciferase reporter gene in NCI-H82. |
| Gene | Luciferase |
| Host Cell | NCI-H82 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: NCI-H82 cells have been instrumental in studying the genetic and molecular changes that occur in mesothelioma, as well as in testing new treatments for the disease. Researchers have used these cells to investigate the role of specific genetic mutations in mesothelioma development and to identify potential therapeutic targets. NCI-H82 cells have also been employed in drug screening assays to evaluate the efficacy of various compounds against mesothelioma cells.
A: NCI-H82 cells exhibit several characteristic features of mesothelioma cells, including a fibroblastic morphology, a propensity for forming tumor spheroids, and a stable growth rate in culture. They also express certain markers associated with mesothelioma, such as cytokeratins and CD117. Additionally, NCI-H82 cells are susceptible to certain drugs and toxins, which makes them useful for studying the efficacy of potential therapeutics.
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