Luciferase Reporter Cell Line - NCI-H82

Name: Luciferase Reporter Cell Line - NCI-H82
SKU: CSC-RR0551
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0551

Host Cell : NCI-H82 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Documents

Cat. No.	CSC-RR0551
Description	This cell line is engineered to stably express luciferase reporter gene in NCI-H82.
Target Gene	Luciferase
Host Cell	NCI-H82
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

CSC-RR0551-Luciferase Reporter Cell Line - NCI-H82-Datasheet

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Background

Applications

Q & A

Customer Reviews

The Luciferase reporter system is a valuable tool in molecular biology research, enabling the study of gene expression and regulation in a sensitive, reliable, and versatile manner. Its applications span various fields, including basic research, drug discovery, and toxicology, making it an essential resource for advancing our understanding of biological processes and developing new therapeutic strategies.

NCI-H82 Cell Line is a human small cell lung cancer cell line that has been used extensively in lung cancer research. It was established from a patient with small cell lung cancer, a highly aggressive and fatal disease, and NCI-H82 cells are invaluable for studying the molecular mechanisms of lung cancer development and testing new anti-cancer drugs. Some of the major advantages of using NCI-H82 cells include rapid growth, ease of culture, and a stable karyotype. These cells also exhibit a high degree of tumourigenicity, making them suitable for preclinical studies of cancer therapies. In addition, NCI-H82 cells can be easily genetically modified, allowing for the study of specific signalling pathways and the development of targeted therapies. In conclusion, the NCI-H82 cell line is an important tool to advance our understanding and treatment of small cell lung cancer.

The Luciferase Reporter NCI-H82 Cell Line is a valuable tool for researchers in the field of lung cancer research. This cell line, derived from the human small cell lung cancer cell line NCI-H82, has been engineered to express the firefly luciferase gene under the control of a promoter of interest.

One of the key advantages of using the Luciferase Reporter NCI-H82 Cell Line is its high sensitivity and specificity in detecting changes in gene expression. The cells are easy to cultivate and have a relatively fast growth rate, providing researchers with a consistent and reliable experimental system. Additionally, the cell line has a stable karyotype, ensuring the consistency of experimental results.

The Luciferase Reporter NCI-H82 Cell Line can be used in various applications, such as studying the effects of different drugs or chemicals on gene expression in lung cancer, investigating the function of specific transcription factors, or examining the regulatory mechanisms of various biological processes. Its versatility and ease of use make it an essential tool for researchers in the field of lung cancer research and a powerful resource for advancing our understanding of gene expression and regulation in this disease.

Customer Q&As

How has NCI-H82 been used in research?

A: NCI-H82 cells have been instrumental in studying the genetic and molecular changes that occur in mesothelioma, as well as in testing new treatments for the disease. Researchers have used these cells to investigate the role of specific genetic mutations in mesothelioma development and to identify potential therapeutic targets. NCI-H82 cells have also been employed in drug screening assays to evaluate the efficacy of various compounds against mesothelioma cells.

What are the characteristics of NCI-H82 cells?

A: NCI-H82 cells exhibit several characteristic features of mesothelioma cells, including a fibroblastic morphology, a propensity for forming tumor spheroids, and a stable growth rate in culture. They also express certain markers associated with mesothelioma, such as cytokeratins and CD117. Additionally, NCI-H82 cells are susceptible to certain drugs and toxins, which makes them useful for studying the efficacy of potential therapeutics.

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