NFAT-Luc Reporter Cell Line-RAW264.7 stably expresses luciferase reporter gene under the control of the NFAT (Nuclear Factor of Activated T-cells) response element. This cell line is an ideal cellular model for studying the NFAT signaling pathway.
The NFAT Leeporter™ cell line is a stably transfected RAW264.7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Nuclear Factor of Activated T-cells (NFAT) response element, so that the cell line is designed to measure the transcriptional activity of NFAT. NFAT is a transcription factor originally found in activated T lymphocytes, and is now known to regulate not only T cell activation and differentiation but also the function of other immune cells including dendritic cells, B cells and megakaryocytes. The NFAT induction by calcium ionophore A23187 is shown in Figure 1.