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Luciferase Reporter Cell Line - NCI-H661

Luciferase Reporter Cell Line - NCI-H661

Cat.No. :  CSC-RR00809 Host Cell:  NCI-H661

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Cat. No. CSC-RR00809
Description This cell line is engineered to stably express Luciferase reporter gene in NCI-H661 cells. It is an ideal cell line model in bioluminescenttracking of NCI-H661 cells.
Gene Luciferase
Host Cell NCI-H661
Host Cell Species Homo sapiens (Human)
Stability This cell line is stable at least 10 passages.
Product Type Stable cell line constitutively expressing Luciferase reporter gene
Applications 1) in vitro cell tracking by bioluminescent signal
2) monitor in vivo tumor growth using the bioluminescence signal
3) anticancer drug development
Quality Control 1) in vitro cell-based luciferase assay
2) mycoplasma detection
Size Form One vial of frozen cells, typically >1x10^6cells/vial
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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