Cat.No. : CSC-RR0516 Host Cell: THP-1
| Cat. No. | CSC-RR0516 |
| Description | This cell line is engineered to stably overexpress luciferase reporter gene. THP-1 is a human leukemia monocytic cell line, which has been widely used in laboratory research. This cell line is a useful tool for bioluminescent tracking of THP-1 cells. |
| Gene | Luciferase |
| Host Cell | THP-1 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The researchers looked at the potential for treating acute myeloid leukemia (AML) with DNA ligands DB1055 and DB818 using the Luciferase Reporter Cell Line - THP-1. This cell line, which is generated from human monocytic leukemia cells, is useful for simulating medication responses and clinical features, making it an essential model for research on AML. By causing AML-specific transcriptome changes, monocyte differentiation into macrophages, and cell growth suppression, DB1055 and DB818 showed strong anti-leukemic effects through in vitro tests. Furthermore, these substances have shown potential in lowering the burden of leukemia in xenograft models obtained from patients, indicating their potential as differentiation treatments that target the HOXA9/DNA interaction. This method provides a fresh way to treat AML, especially in instances when HOXA9 overexpression and chemotherapy resistance are present.
Figure 1. The researchers generated THP-1-Luc cells using a lentivirus expressing luciferase and transduced them with lentiviruses expressing shRNAs (shHOXA9-1F2/-1F3 or shCTR). (Lambert M, et al., 2024)
A: The reporter gene stability of THP-1 Luciferase Reporter Cell Line is a key factor that determines the reliability of experimental results. This cell line has been tested for multiple generations to ensure that the luciferase reporter gene is stably integrated into the genome and is not easily lost. Ensuring the stability of gene expression can make experimental data more reproducible and consistent in different experiments and reduce errors caused by fluctuations in gene expression.
A: THP-1 Luciferase Reporter Cell Line is optimized for highly sensitive detection of luciferase activity. As a reporter gene, luciferase's expression level is related to experimental treatment conditions, and it can quickly and accurately reflect changes in intracellular biochemical processes through a fluorescence detection system. Its high sensitivity can detect small biological changes, making experimental results more precise.
A: THP-1 Luciferase Reporter Cell Line is widely used in drug screening, especially in the field of immune response and inflammation research. The introduction of the luciferase reporter gene allows the effect of drugs on intracellular signaling pathways to be evaluated through changes in luciferase activity. Experimental data show that this cell line shows good sensitivity and specificity in detecting drug effects, toxicity assessment and mechanism research, and can provide reliable data support for drug development.
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The logistics speed is very fast, the packaging is also very tight, and the product is in good condition when received. The cell state is very stable. During the culture process, I observed consistent cell growth and gene expression, and the experimental data was also very consistent. The results of using THP-1 cells to conduct research on acute monocytic leukemia are particularly reliable, which really saves a lot of time and energy. This shopping experience is very satisfying and worth recommending!
The THP-1 cell line is very stable in culture and has consistent gene expression. Compared with PBMCs, THP-1 cells are easier to culture, the experiments are highly reproducible, and the variables caused by individual differences are reduced. Especially after adding the luciferase reporter gene, it becomes very intuitive and convenient to observe the changes in the signaling pathway. I will continue to use this cell line for immune response and inflammation research.
Thank you very much for the excellent service of the merchant. The cells maintained good activity during transportation. It is not difficult to culture THP-1 cells, their physical and functional characteristics are very close to primary human monocytes, and the experimental results are more convincing. Using a luciferase reporter gene allows us to track changes in signaling pathways and gene expression in real time, making our research more efficient. This purchasing experience was very pleasant, and the service provided by the merchant was also very professional.
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