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Luciferase Reporter Cell Line - NIH/3T3

Luciferase Reporter Cell Line - NIH/3T3

Cat.No. :  CSC-RR0376 Host Cell:  NIH/3T3

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Cell Line Information

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Cat. No. CSC-RR0376
Introduction NIH/3T3-Luc cell line is constructed by stable integration of luciferase encoding gene into NIH/3T3 cell genome. It is a good cell model for in vitro or in vivo cancer research.
Gene Luciferase
Host Cell NIH/3T3
Host Cell Species Mus musculus (Mouse)
Stability Validated for at least 10 passages
Application

1. Gene expression studies

2. Protein localization

3. Drug screening and toxicology

4. Live cell imaging

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Shipping Dry ice
Storage Liquid nitrogen
Source NIH/3T3
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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