Cat.No. : CSC-RR0547 Host Cell: Neuro2a
| Cat. No. | CSC-RR0547 |
| Description | This cell line is engineered to stably overexpress luciferase reporter gene in Neuro2a. |
| Gene | Luciferase |
| Host Cell | Neuro2a |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The urokinase receptor (uPAR), encoded by the Plaur gene and also known as CD87, U-PAR, and UPAR, exerts diverse physiological and pathological effects. The purpose of this study was to better understand the role of novel small RNAs encoded within the Plaur gene, Plaur-miR1-3p and Plaur-miR1-5p, and their regulatory effects on target genes, Mef2d and Emx2. The researchers used Neuro2a cells as a model to validate the Plaur-dependent expression of these RNAs and their regulatory effects on subsequent Mef2d and Emx2 target genes. Bioinformatic analysis identified potential binding sites and subsequent Luciferase reporter assays confirmed Plaur-miR1-5p's activating effect on Mef2d expression. Furthermore, sequencing of mouse brain samples revealed additional targets, Nrip3 and Snrnp200, suggesting a broader regulatory role of these small RNAs in neural tissues.
Figure 1. The researchers utilized the Luciferase Reporter Cell Line - Neuro2a to conduct a luciferase reporter assay, confirming the direct activation of Mef2d expression by Plaur-miR1 through 3′-UTR interaction. (Rysenkova KD, et al., 2022)
A: Luciferase Reporter Cell Line - The luciferase reporter in Neuro2a is highly sensitive and capable of detecting small biochemical changes within cells. The intensity of the light signal of luciferase is directly related to the expression level of the reporter gene, and its activity can be measured quickly and accurately through a fluorescence detection system. This high sensitivity is particularly important for signaling pathway analysis and drug screening in neuroscience research.
A: Luciferase Reporter Cell Line - Neuro2a is specially designed for neuroscience research. Neuro2a cell line is derived from mouse neuroblastoma and has the characteristics of nerve cells. Combined with the luciferase reporter gene, it can be used to study signal transduction, gene expression regulation and neurotoxicity of nerve cells. It performs well in studies of neuronal differentiation, synaptic plasticity and neurodegenerative disease models, providing reliable data support.
A: Luciferase Reporter Cell Line - Neuro2a is ideal for high-throughput screening experiments. The introduction of the luciferase reporter gene allows the changes in luciferase activity after drug treatment to be quickly read through a high-throughput fluorescence detection system. This cell line is widely used in drug screening, toxicity assessment and gene function research. It can improve experimental efficiency and provide a high-throughput and high-sensitivity screening platform.
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My recent acquisition of Luciferase Reporter Cell Line - Neuro2a has left me quite happy. First of all, the trial proceeded nearly without delay due to the lightning-fast logistics. To guarantee that the cells are active, the product is packed securely. Consistent bioluminescence was seen during operation, allowing for the real-time observation of biological activities and the provision of useful data for research. This tool is a real help while conducting cell tests.
The bought Luciferase Reporter Cell Line, Neuro2a, functions effectively. We cultivated the cells in accordance with the guidelines after obtaining them. Throughout the development phase, the cells were remarkably steady and no anomalies emerged. Luciferase exhibits exceptional performance, particularly with regard to gene expression; bioluminescence is easily observable, and the experimental data is highly trustworthy. This is an extremely affordable cell line for toxicology and neurology research.
The Luciferase Reporter Cell Line - Neuro2a that I utilized this time really pleased me. First of all, the merchant has excellent customer service and provides prompt, thorough answers to inquiries. Second, after being received, the cells were in good shape and had been properly packaged and transported. It is especially important to note how well these cells performed in the experiment, particularly with regard to the synthesis and assembly of tubulin. The experiment went well, and the data were quite clear. This product is highly recommended for study, whether it be applied or basic.
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