Luc Reporter Cell Line-CT26

Case Study 1

Here,researchers grafted murine colon carcinoma CT-26 cells expressing luciferase into immunocompetent BALB-c mice by intravenous injection(IV group),subcutaneous injection(SC group),intraperitoneal injection after peritoneal scratching(A group)or intraperitoneal injection alone(IP group).Tumour growth was monitored by bioluminescence during the first 15 days post-grafting.Studies have shown that limited peritoneal metastasis(PM)was obtained by intraperitoneal injection,whereas intraperitoneal injection followed by peritoneal scraping produced a mouse model of extensive PM for the evaluation of new therapies.

Figure 1.In vivoquantification of tumor growth by bioluminescence tracking of luciferase expressing CT-26 cells(CSC-RR0237,Creative Biogene,Shirley,NY,USA)over 15 days.(Taibi A,et al.2019)

Case Study 2

The application of nanosecond pulsed electric fields(nsPEF)may be an effective therapeutic strategy for peritoneal metastasis(PM)of colorectal cancer(CRC).The purpose of this study was to evaluate the sensitivity of CT-26 CRC cells to nsPEF combined with chemotherapeutic agents in vitro and to observe the subsequent histological responseIn vivo.

Figure 2.nsPEFs-induced immediate dose-dependent cell death(a,b)and growth inhibition(c,d)in CT26-Luc reporter cell(CSC-RR0237,Creative Biogene,NY,USA)in vitro in a 1-mm cuvette system.(Taibi A,et al.2021)

Figure 3.nsPEF stimulation-induced permeabilization and intracellular calcium fux in CT-26 cells(CSC-RR0237,Creative Biogene,NY,USA)with 300-µm bi-electrode system.Fluorescence intensity changes(ΔF/F0)of YO-PRO(a,b)and Fluo4(c,d)in CT-26 cells were measured using live confocal microscopy,every 3 s for 3 min.(Taibi A,et al.2021)

Figure 4.Swelling of mitochondria was immediately observed in CT-26 tumor cells(CSC-RR0237,Creative Biogene,NY,USA)in mice after nsPEF exposure in vivo with 2-mm bi-electrodes.(Taibi A,et al.2021)

Case Study 3

This study describes the development of a novel bacteria-mediated tumor therapy(BMT)approach based on an attenuated strain of S.typhimurium that secretes a fusion protein consisting of FlaB and IL15 that synergizes to enhance antitumor activity and long-term immune memory in mouse tumor models.Furthermore,to examine the enhancing effects of BMT and ICI,mice with highly metastatic tumors were treated with a combination of these bacteria and anti-PD-L1 antibodies.These findings may provide new insights into the utilization of BMT and ICI,suggesting a promising combined approach for cancer immunotherapy.

Figure 5.Antitumor effect and immune memory response by SAM secreting IL15/FlaB in mice bearing CT26 subcutaneous(sc)tumors.(Zhang Y,et al.2023)

To establish mouse tumor models,MC38(1×10⁶cells in 100μL PBS)or B16F10(5×10⁵cells in 100μL PBS)tumor cells were subcutaneously implanted into the right flank of C57BL/6 mice.In addition,CT26(1×10⁶in 100μL PBS),CT26-Luc(1.2×10⁶in 100μL PBS)(CSC-RR0237,Creative Biogene,NY,USA),or 4T1-Luc2(8×10⁶in 100μL PBS)cells were implanted into BALB/c mice.