Luciferase Reporter Cell Line - KU-812E

Name: Luciferase Reporter Cell Line - KU-812E
SKU: CSC-RR0582
Rating: 4.0 (3 reviews)

Cat.No. : CSC-RR0582 Host Cell: KU-812E

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Cell Line Information

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Cat. No.	CSC-RR0582
Description	This cell line is engineered to stably express luciferase reporter gene in KU-812E cells.
Gene	Luciferase
Host Cell	KU-812E
Host Cell Species	Homo sapiens (Human)
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Quick Inquiry

Background

Applications

Publications

Q & A

Customer Reviews

KU-812E, derived from a 38-year-old male patient with chronic myeloid leukemia in 1981, is a human peripheral blood basophilic leukemia cell line. This cell line expresses CD3 and ANPEP antigens while lacking lymphoid markers. It harbors at least one Ph1 chromosome and exhibits characteristics of basophilic leukocytes, expressing abundant proteins associated with basophils and eosinophils, such as mast cell tryptase, carboxypeptidase A, high-affinity immunoglobulin E (IgE) receptors alpha and gamma chains, as well as core proteins involved in heparin and chondroitin sulfate synthesis. Additionally, it secretes Fc receptors, basophilic granules, histamine, and other mediators.
KU-812E cells possess a primitive granulocytic morphology and grow in suspension with a doubling time of 20-30 hours. Due to its diverse functional characteristics, KU-812E is extensively utilized in immunological research and serves as an in vitro model for studying the interaction and function of IL-33/ST2L based on human cell lines. STR profiling confirms the identity of KU-812E cells, and they test negative for mycoplasma contamination. Recommended culture conditions include the use of RPMI 1640 medium supplemented with 10% fetal bovine serum at 37°C in a 5% CO2 humidified atmosphere. Serum-free culture conditions can induce differentiation. Maintenance of optimal cell conditions involves regular media changes (2-3 times per week) and subculturing at a ratio of 1:2 to 1:4. Cell density should not exceed 3 x 10^6 cells/ml, and low-density cultures can be supplemented with fresh medium as needed. Luciferase reporter cell line KU-812E is engineered to express a luciferase reporter gene for monitoring cellular processes.

Gene regulation research: Luciferase Reporter Cell Line - KU-812E is not only suitable for monitoring gene expression levels, but also can be used to study transcription factor activity, DNA methylation and chromatin structure changes. By introducing different promoters or response elements, different gene regulatory networks can be simulated to deeply explore the complexity of intracellular gene regulatory mechanisms.

Immune regulation mechanism research: KU-812E cell line plays an important role in simulating immune response and inflammation. By activating or inhibiting specific signaling pathways, the production of immune regulatory factors, the release of cytokines, and the activation and proliferation of immune cells can be studied, providing a theoretical basis for the treatment of inflammatory diseases and autoimmune diseases.

Leukemia drug screening: KU-812E cell line is representative of the sensitivity and resistance to leukemia-related drugs, and can be used to screen new drugs targeting leukemia cells and evaluate the toxic side effects of drugs. At the same time, combined with luciferase reporter gene technology, high-throughput drug screening and evaluation can be achieved.

Cell signal transduction research: Using KU-812E cell line, the cross-regulation and interaction mechanism of intracellular and extracellular signal transduction pathways can be studied. By intervening in the activity of specific signaling pathways, we can explore physiological processes such as cell proliferation, differentiation, and apoptosis, and provide new ideas and targets for the development of tumor treatment and immunomodulatory therapy.

Luciferase activity detection: As a highly sensitive biomarker detection method, luciferase activity detection can be widely used in the fields of cell biology, molecular biology, and drug screening. As a cell model that stably expresses luciferase, the KU-812E cell line provides an effective tool and platform for rapid and accurate detection of cell activity, toxicity, and drug efficacy.

Customer Q&As

What is the luciferase reporter gene integration strategy for the KU-812E cell line?

A: Integration is usually carried out through the use of retroviral or lentiviral vectors, which can stably insert the luciferase gene into specific sites in the cell genome, ensuring long-term stable expression. Selecting specific sites for integration can avoid the risk of insertional mutations and ensure consistent expression of the luciferase gene under different experimental conditions.

How do the characteristics of the KU-812E cell line affect experimental design?

A: The KU-812E cell line is a human leukemia cell line whose characteristics make it particularly suitable for hematology-related research. The integration of luciferase reporter genes allows researchers to easily evaluate the effects of different drugs or gene regulation on cell proliferation, apoptosis, and signaling pathways. For example, by adding specific activators or inhibitors, changes in signaling pathway activity can be monitored in real time and potential therapeutic drugs can be screened.

What issues should be paid attention to when culturing KU-812E cells?

A: When culturing KU-812E cells, attention should be paid to the selection of culture medium and the control of cell density. RPMI-1640 medium containing 10% fetal calf serum is usually used and maintained at 37°C and 5% CO2. In addition, cell density that is too high or too low may affect luciferase expression and experimental results, so regular checking of cell status and timely passage are key to ensuring experimental success.

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Customer Reviews

Short doubling time

First, the logistics was fast and the cells arrived in good condition. During the culture process, the cells showed typical granulocyte morphology, grew in suspension, and proliferated rapidly (doubling in 20-30 hours). Through the Luciferase reporter gene, I can monitor biological processes in cells in real time, especially in the study of the IL-33/ST2L signaling pathway, and the data is very reliable. I recommend this cell line to colleagues doing immune research.

Germany

05/19/2021

Rich protein expression

Experiments were conducted using Luciferase Reporter Cell Line - KU-812E, and the results were satisfactory. Cells grow stably. Pay attention to maintaining an appropriate cell density during suspension culture and replace the culture medium in a timely manner to maintain optimal conditions. The KU-812E cell line performs well in studying the expression of basophil- and eosinophil-related proteins and is particularly suitable for research on immunology and cell-cell interactions.

Germany

01/31/2022

Monitoring is intuitive

This time I used Luciferase Reporter Cell Line - KU-812E for cell research, and the overall experience was very good. . KU-812E cells express a large number of proteins related to basophils and eosinophils, and are suitable for studying the secretion mechanism of mediators such as Fc receptors and histamine. The introduction of the Luciferase reporter gene makes monitoring cellular processes more intuitive and convenient.

United Kingdom

08/31/2021

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Luciferase Reporter Cell Line - KU-812E

Cell Line Information

Cell Culture Information

Safety and Packaging

Documents

Background

Applications

Publications

Q & A

Customer Reviews

What is the luciferase reporter gene integration strategy for the KU-812E cell line?

How do the characteristics of the KU-812E cell line affect experimental design?

What issues should be paid attention to when culturing KU-812E cells?

Short doubling time

Rich protein expression

Monitoring is intuitive

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