HIF Luciferase Reporter Cell Line - Cos-7

Name: HIF Luciferase Reporter Cell Line - Cos-7
SKU: CSC-RR0396
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0396

Host Cell : COS-7 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RR0396
Introduction	HIF Luciferase Reporter Cell Line - Cos-7 is a recombinant monkey fibroblast cell line which stably expresses luciferase reporter gene under the control of HIF response element. It is designed for monitoring the activation of hypoxia pathway triggered by stimuli.
Target Gene	Luciferase
Host Cell	COS-7
Host Cell Species	Chlorocebus sabaeus (Green monkey)
Source	Cos-7
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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