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Cat.No. : CSC-RR0601 Host Cell: SU-DHL-4
| Cat. No. | CSC-RR0601 |
| Description | This cell line is engineered to stably express luciferase reporter gene in SU-DHL-4 cells. |
| Gene | Luciferase |
| Host Cell | SU-DHL-4 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The researchers investigated the prognostic impact of miR-21, miR-17-92, and miR-155 in diffuse large B-cell lymphoma (DLBCL) patients, utilizing quantitative reverse-transcription-PCR to analyze their expression levels in DLBCL tissues (n=200) versus control tonsils. They found significant up-regulation of miR-21, miR-17-92, and miR-155 in DLBCL tissues. High miR-21 and miR-17-92 levels correlated with shorter progression-free and overall survival. MiR-21 was identified as an independent prognostic factor in DLBCL patients receiving rituximab-combined chemotherapy. Experimental methods included luciferase reporter assays using Luciferase Reporter Cell Line - SU-DHL-4 cells to demonstrate miR-21's direct targeting of FOXO1 and PTEN, leading to inhibition of Bim transcription and activation of the PI3K/AKT/mTOR pathway. Moreover, miR-21 inhibition suppressed MDR1 expression and sensitized DLBCL cells to doxorubicin, suggesting therapeutic potential in DLBCL treatment strategies.
Figure 1. The researchers assessed miR-21's impact on FOXO1 and PTEN expression in DLBCLs through qRT-PCR and luciferase assays using Luciferase Reporter Cell Line - SU-DHL-4. They transfected inhibitors/mimics and measured gene expression and luciferase activity to explore transcriptional regulation. (Go H, et al., 2015)
A: The luciferase reporter gene in the SU-DHL-4 cell line showed sensitive and predictable responses under different experimental conditions. For example, upon addition of an activator or inhibitor of a specific signaling pathway, luciferase activity will change significantly, reflecting changes in the activity of the corresponding pathway. This sensitivity makes it an ideal tool for studying cell signaling and drug screening.
A: SU-DHL-4 cell line, as a human lymphoma cell line, has important application value in tumor research. The integration of luciferase reporter genes makes it a powerful tool for studying tumor signaling pathways, evaluating the effects of anticancer drugs, and exploring changes in the tumor microenvironment. By detecting luciferase activity, the effects of drugs on tumor cell proliferation and apoptosis can be quickly assessed and potential therapeutic drugs can be screened.
A: When culturing the SU-DHL-4 cell line, special attention needs to be paid to the preparation of the culture medium and the control of culture conditions. RPMI-1640 medium containing 10% fetal calf serum is usually used and maintained at 37°C and 5% CO2. In addition, excessive density or sparseness should be avoided when cells are passaged to ensure stable expression of the luciferase reporter gene and accuracy of experimental results. Regular checks of cell status and timely adjustments to culture conditions are critical to the success of the experiment.
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The product logistics of this Luciferase reporter cell line-SU-DHL-4 is awesome! I received it very quickly and it was packaged very carefully without any damage. From order to receipt, the whole process was very smooth. I feel like this kind of service makes me feel very reassured and makes me more willing to buy again.
Germany
01/18/2023
The quality of the product is very reliable and I can easily conduct research on gene expression regulation and signaling pathways. Whether it is my personal experiments or team projects, I have received great help. Thanks for providing such a convenient tool for our research!
French
02/05/2023
My feelings about this Luciferase reporter cell line-SU-DHL-4 can be described in one word: stable. The quality of this product is very stable, cell growth has been maintained in a good state, and gene expression is also very stable. In my experiments, I observed rapid cell growth and significant gene expression. This gave me very accurate data and made my research more reliable.
United Kingdom
05/24/2022
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