Luc Reporter Cell Line-B16F10

Name: Luc Reporter Cell Line-B16F10
SKU: CSC-RR0234
Rating: 4.0 (3 reviews)

Cat.No. : CSC-RR0234 Host Cell: B16F10

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RR0234
Description	Luc Reporter Cell Line-B16F10 is engineered to stably overexpress luciferase reporter gene. It is an ideal positive control for use in bioluminescence assays. In addition, B16F10 cells can form metastases in mouse lungs. The in vivo growth of metastases can be monitored using bioluminescent imaging.
Gene	Luciferase
Host Cell	B16F10
Host Cell Species	Mus musculus (Mouse)
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Case Study

Applications

Publications

Q & A

Customer Reviews

B16-F10 is a subclone that was generated from mouse melanoma cells B16, which were isolated from melanoma tissue of C57BL/6J mice. Its mixed morphology is epithelial-like and spindle-shaped. At The Jackson Laboratory in the United States, tumors that spontaneously emerged on the ear skin of C57BL/6J mice were used to develop the mouse melanoma cell line B16 in 1954.
Because B16-F10 cells can spread quickly to the lungs when given intravenously to mice, they are frequently employed in cancer research. They are frequently used to investigate different facets of tumor growth, metastasis, and treatment strategies. These cells are frequently used to study the molecular pathways causing melanoma metastasis because of their great capacity for metastasis. Standard cell culture conditions are used to cultivate B16-F10 cells. Fetal bovine serum (FBS) and antibiotics are added to Dulbecco's Modified Eagle Medium (DMEM) for maintenance. They proliferate quickly in vitro and stick nicely to tissue culture plates. Through genetic modification, the B16-F10 cells express a luciferase reporter gene, allowing for non-invasive bioluminescence imaging tools to monitor tumor growth and metastasis in vivo.

Malignant melanoma, though less common among skin cancers, accounts for the highest mortality rate associated with these malignancies. Using Gli1 knockdown (KD) Luc Reporter Cell Line-B16F10 cells, the researchers examined the role of GLI1 in melanoma cell invasion. GLI1, a component of sonic hedgehog pathways, was found to be crucial for maintaining invasive and mesenchymal properties independent of MITF. Gli1 KD cells demonstrated decreased lung metastasis in mice, along with a shift from mesenchymal-to-epithelial transition and downregulation of transcription factors that induce epithelial growth (EMT) Snail1, Zeb1, and Twist1. These results illustrate the role of GLI1 in controlling melanoma cell plasticity and heterogeneity, providing insight into the mechanisms underlying melanoma progression.

Figure 1 depicts the experimental findings on the blockade of Sonic hedgehog (Shh) signaling and its effect on melanoma cell invasion. (doi: 10.1111/cas.13294) Figure 1. Luc Reporter Cell Line-B16F10 was used by the researchers to show how gene expression analysis using qRT-PCR, western blotting, luciferase assays, and Matrigel invasion assays evaluate the effect of Shh signaling inhibition on melanoma invasion. (Gunarta IK, et al., 2017)

Tumor growth and metastasis research: B16-F10 cells are a commonly used mouse melanoma cell line that has the ability to quickly metastasize to mouse lungs, so they are widely used in cancer research. Using this feature, issues such as tumor growth, metastasis, and treatment strategies can be studied.

Molecular pathway regulation research: Since B16-F10 cells have good metastatic ability, they can be used to study the molecular pathways and signaling pathways that lead to melanoma metastasis, providing new targets and strategies for the treatment of melanoma.

Bioluminescence imaging technology: Through genetic modification, B16-F10 cells express luciferase reporter genes, and bioluminescence imaging technology can be used to non-invasively monitor tumor growth and metastasis in vivo, providing convenience for dynamic observation in vivo.

Drug screening and evaluation: Using the B16-F10 cell model, drug screening and evaluation experiments can be performed to evaluate the effects of candidate drugs on melanoma growth and metastasis, providing important references for new drug development and clinical treatment.

Tumor immunotherapy research: B16-F10 cells can be used to study the effectiveness and mechanism of tumor immunotherapy strategies, including immune checkpoint inhibitors, CAR-T cell therapy, etc., to provide a theoretical basis for improving the treatment effect of melanoma patients.

Customer Q&As

Luc Reporter Cell Line-B16/F10 What is the Luciferase expression level of the Reporter cell line? Is it stable under different culture conditions?

A: Our Luc Reporter Cell Line-B16/F10 has undergone strict screening and verification to ensure the stable expression of the Luciferase gene. Through methods such as luciferase activity detection and PCR, we verified the expression level of Luciferase and performed repeated tests at different time points and in different batches. Cells can maintain stable Luciferase expression under appropriate culture conditions.

How does the B16/F10 background affect the performance of this Reporter cell line? Are special culture conditions required?

A: The impact of the B16/F10 cell line as a vector on the Luc Reporter cell line is considered minimal. However, we recommend that customers pay attention to the special growth requirements of the B16/F10 cell line during use, such as culture medium components and culture conditions, to ensure optimal expression and stability of the cells. We provide detailed training guides to help clients overcome potential issues.

Have the cell lines for this product been tested for contaminants? How to ensure the purity of cell lines?

A: Yes, our Luc Reporter Cell Line-B16/F10 has undergone rigorous contaminant testing, including testing for common contaminants such as mycoplasma and bacteria. We use methods such as PCR and cell culture testing to ensure the purity of our cell lines. Our strict quality control procedures ensure that each batch of cell lines meets high standards of purity.

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Customer Reviews

Stable and consistent

Cell stability is excellent, with consistent growth and stable gene expression seen in every culture. I particularly like their ability to proliferate quickly and the cells to adhere firmly to the wall. Moreover, non-invasive bioluminescent imaging of tumor growth and metastasis in vivo can be easily performed by genetically modifying the expression of a luciferase reporter gene. These cells are particularly useful in cancer research, particularly studying the mechanisms of metastasis in melanoma.

Canada

04/04/2023

Fast growth

They demonstrate excellent stability and consistency in routine cell culture. Especially in lung metastasis studies, these cells perform extremely well. They can be seen quickly spreading to the lungs with every use, making them the best tool for studying tumor metastasis. Moreover, the genetically modified luciferase reporter gene makes monitoring tumor growth simpler and more intuitive.

Canada

10/19/2020

Easy to monitor

They exhibit excellent growth rates and stable gene expression during culture. Moreover, they adhere very firmly to the wall and are suitable for various experimental operations. What surprises me most is that the introduction of the luciferase reporter gene makes monitoring tumor growth and metastasis in the body very convenient and efficient. This cell line is extremely valuable in studying the mechanisms of melanoma metastasis.

United Kingdom

01/08/2023

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Luc Reporter Cell Line-B16F10

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Case Study

Applications

Publications

Q & A

Customer Reviews

Luc Reporter Cell Line-B16/F10 What is the Luciferase expression level of the Reporter cell line? Is it stable under different culture conditions?

How does the B16/F10 background affect the performance of this Reporter cell line? Are special culture conditions required?

Have the cell lines for this product been tested for contaminants? How to ensure the purity of cell lines?

Stable and consistent

Fast growth

Easy to monitor

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