Luciferase Reporter Cell Line - U937

U937 cells were established by Sundstrom and Nilsson in 1976 from the pleural effusion of a 37-year-old white man with systemic histiocytic lymphoma and represent a unique promonocytic phenotype. These cells embody the typical morphology of the mononuclear macrophage cell type, with a rounded structure, an average diameter of approximately 14 μm, and surface markers such as the human DC-SIGN protein, which are critical for their role in immunological studies. Due to the relative homogeneity and low maintenance requirements of expanded cultures, these cells have since been used as an important tool for studying phagocyte differentiation and different types of cell-cell interactions. U-937 cells mature and differentiate in response to a variety of soluble stimuli, adopting the morphology and characteristics of monocytes, macrophages, or dendritic cells. Through techniques such as 3D cell culture and advanced imaging, U937 cells provide a near-vivo environment that allows researchers to observe cell behavior and interactions in a more physiologically relevant environment. The luciferase reporter cell line - U937 is an innovative tool widely used in biomedical research. This cell line was engineered to stably overexpress a luciferase reporter gene in U937. Luciferase is an enzyme that generates light in the presence of its substrate luciferin, providing bioluminescent readouts for a variety of cellular processes. Any changes in luciferase activity can be easily measured using a photometer, making this cell line not only efficient but also convenient for real-time monitoring of gene expression. Therefore, this cell line can be used as a reporter system to analyze the activation or inhibition of specific signaling pathways or transcription factors.