NFAT Luciferase Reporter Cell Line - Jurkat

Name: NFAT Luciferase Reporter Cell Line - Jurkat
SKU: CSC-RR0389
Rating: 4.0 (3 reviews)

Cat.No. : CSC-RR0389 Host Cell: Jurkat

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RR0389
Introduction	NFAT Luciferase Reporter Cell Line - Jurkat is a recombinant human T lymphocyte which stably expresses luciferase reporter gene under the control of NFAT response element. It is designed for monitoring the activation of NFAT signaling pathway triggered by stimuli.
Gene	Luciferase
Host Cell	Jurkat
Host Cell Species	Homo sapiens (Human)
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen
Source	Jurkat

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Case Study

Applications

Publications

Q & A

Customer Reviews

NFAT Luciferase Reporter Cell Line: The luciferase reporter gene is expressed consistently in Jurkat, a recombinant human T cell, by means of the NFAT response element. The transcription factors known as NFAT (Nuclear Factor of Activated T-Cells) are essential for the growth and operation of the immune system. NFAT isoforms have been discovered as five, including NFAT5 and NFATC1-C4.
This cell line is an invaluable resource for tracking the NFAT signaling pathway's activity in response to different stimuli. Both an NFAT-homology region (NHR) and a REL-homology region (RHR) are found in NFAT proteins. When the T cell receptor (TCR) is stimulated, they move from their original location in the cytosol to the nucleus. NFAT proteins are essential for stimulating gene transcription during immune responses because they are a component of the nuclear factors of activated T cells transcription complex in the nucleus.
Jurkat cells are frequently employed in immunological research because of their T cell origin and ease of manipulation in vitro. They are produced from a human T lymphocyte leukemia. They have traits common to T lymphocytes and grow well in suspension culture.

YIV-906 is a cancer drug based on a traditional Chinese medicine formula that has shown the potential to prolong survival and improve quality of life in cancer patients in multiple clinical studies. As an immunomodulator in the tumor microenvironment, YIV-906 can enhance the anti-tumor activity of multiple anticancer drugs, reduce gastrointestinal side effects, and accelerate the recovery of damaged tissues. The experimental method included in the Jurkat cell-Staphylococcus aureus superantigen E (SEE)-Raji cell culture model, YIV-906 promoted T cell activation by enhancing NFAT activity and upregulating CD69. YIV-906 can trigger phosphorylation of TCR downstream signaling cascades without TCR involvement and inhibit the activity of SHP1 and SHP2, both of which dephosphorylate TCR downstream proteins under PD1-PD-L1 interaction. YIV-906 enhances the suppressed NFAT activity under PD1-PD-L1 interaction by inhibiting the activity of SHP1/2. In addition, YIV-906 also enhances the killing ability of Jurkat cells against CD19-expressing cells (such as Raji cells) when expressing chimeric antigen receptor (CAR-CD19-CD3z), regardless of whether PD1-PD-L1 is overexpressed.

Figure 1 depicts the effects of YIV-906 on TCR downstream protein phosphorylation and NFAT-mediated transcriptional activity in Jurkat cells, with emphasis on the presence or absence of SHP1 and/or SHP2. (doi: 10.3389/fphar.2022.1095186) Figure 1. To assess NFAT-mediated transcriptional activity and downstream TCR protein phosphorylation, especially in relation to SHP1 and SHP2 enzymatic activities and knockouts, the researchers employed the NFAT Luciferase Reporter Cell Line - Jurkat. (Lam W, et al., 2023)

Immune response study: Using the NFAT Luciferase Reporter cell line Jurkat, study the activity of the NFAT signaling pathway under different stimuli to reveal its role in the immune system, especially the gene transcription regulation mechanism after T cell receptor (TCR) activation.

Drug screening and evaluation: Through the Jurkat cell model, test and evaluate drugs that affect the NFAT signaling pathway, screen potential immunomodulators and anti-inflammatory drugs, and promote new drug development.

Gene expression study: Using the NFAT Luciferase Reporter cell line, study the gene expression regulation of NFAT protein in immune response, especially its nuclear transfer and transcription complex formation process when activating T cells.

T cell function study: Using the T cell characteristics of Jurkat cells, explore the specific role of the NFAT signaling pathway in T cell growth, differentiation and function, and understand its key role in the immune system.

Signal transduction research: By studying the role of NFAT homology region (NHR) and REL homology region (RHR), the mechanism of NFAT protein in the process of cytoplasm-to-nucleus transfer is revealed, providing a basis for a deeper understanding of T cell signal transduction.

Customer Q&As

NFAT Luciferase Reporter Cell Line - What is the Luciferase expression level of the Jurkat cell line? Are there fluctuations in gene expression levels?

A: Our NFAT Luciferase Reporter cell line has been rigorously screened and verified to ensure stable expression of Luciferase. We verified the expression level of Luciferase through methods such as luciferase activity detection and PCR, and repeated tests were performed at different time points and in different batches. Cells express Luciferase under appropriate culture conditions and maintain stable expression levels.

How does the Jurkat background of this Reporter cell line affect the performance of the product? Are there any special considerations related to this cell line?

A: Our NFAT Luciferase Reporter cell line has demonstrated excellent performance in the Jurkat cell line. During use, we recommend that customers pay close attention to the growth of cells and follow the culture guidelines we provide. Our cell lines have been optimized to ensure optimal expression and stability in the Jurkat cell line.

Has the Jurkat cell line been screened and optimized for this product? How does the screening process work?

A: Yes, our Jurkat cell line has undergone a rigorous screening and optimization process. We use specific screening criteria, including luciferase activity assays and gene expression levels, to ensure that the stability and expression levels of the cell lines meet our quality standards. We also performed long-term culture and expansion of the cell line to verify its stability and reproducibility under different conditions.

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Customer Reviews

Stability

This NFAT Luciferase Reporter cell line shows excellent stability in Jurkat cells. In my culture experiments, I particularly observed continued cell growth and stability of gene expression. The cells showed a consistent response regardless of the stimulus conditions, and I was very pleased with their performance. During the research process, I found that these cells are very useful for detecting the activity of the NFAT signaling pathway, and can provide reliable data support whether in basic research or drug screening.

United Kingdom

01/16/2022

Consistency

The expression profile of the NFAT Luciferase Reporter cell line in Jurkat cells is impressive. In my experiments, I observed that these cells responded very consistently to different stimuli, maintaining stable levels of gene expression both during culture and under changes in experimental conditions. This provides great convenience for our research, allowing us to more accurately evaluate the activity of the NFAT signaling pathway and further explore the function and regulatory mechanism of the immune system.

United States

01/28/2021

Reliability

The application effect of NFAT Luciferase Reporter cell line in Jurkat cells is very significant. In my experiments, I observed that these cells responded to different stimuli with high consistency, and they could stably express the Luciferase reporter gene whether during short-term stimulation or long-term culture. This provides a reliable data basis for our research, enables us to have a deeper understanding of the mechanism of the NFAT signaling pathway in immune response, and provides an important reference for related drug development.

United States

01/26/2023

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NFAT Luciferase Reporter Cell Line - Jurkat

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Case Study

Applications

Publications

Q & A

Customer Reviews

NFAT Luciferase Reporter Cell Line - What is the Luciferase expression level of the Jurkat cell line? Are there fluctuations in gene expression levels?

How does the Jurkat background of this Reporter cell line affect the performance of the product? Are there any special considerations related to this cell line?

Has the Jurkat cell line been screened and optimized for this product? How does the screening process work?

Stability

Consistency

Reliability

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