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HIV-1 Luc Reporter Cell Line - CEM

HIV-1 Luc Reporter Cell Line - CEM

Cat.No. :  CSC-RR0458 Host Cell:  CEM

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Cell Line Information

Cell Culture Information

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Cat. No. CSC-RR0458
Introduction This cell line is designed to monitor Human Immunodeficiency Virus (HIV) infection. A construct containing HIV LTRs, luciferase encoding gene and Rev-Response Element (RRE) is stably integrated into genome of CEM (CD4+, CXCR4+) human CD4 T-cells. Luciferase expression can be simply detected in the presence of HIV.
Gene Luciferase
Host Cell CEM
Host Cell Species Homo sapiens (Human)
Stability Validated for at least 10 passages
Application

1. Gene expression studies

2. Protein localization

3. Drug screening and toxicology

4. Live cell imaging

Applications 1) HIV studies2) Anti-HIV drug screening
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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