Cat.No. : CSC-RR0558 Host Cell: T84
| Cat. No. | CSC-RR0558 |
| Description | This cell line is engineered to stably overexpress luciferase reporter gene in T84 cells. |
| Gene | Luciferase |
| Host Cell | T84 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The Hippo pathway plays a crucial role in maintaining tissue balance, regulating organ size, and influencing tumorigenesis. In order to determine the gene regulatory regions and transcription factors essential for intestinal expression, the researchers used the Luciferase Reporter Cell Line - T84 to study the transcriptional regulation of YAP1 in intestinal cells. Via bioinformatic research, intragenic enhancers in the YAP1 gene may exist in CDX2 and HNF4α chromatin immunoprecipitated DNA from differentiated Caco-2 cells. The researchers verified one strong enhancer of YAP1 promoter activity by transfecting Caco-2 and T84 cells with luciferase-expressing YAP1 promoter-reporter constructs including these enhancer sites. Using an electrophoretic mobility shift assay and chromatin immunoprecipitation assays in Caco-2 cells, in silico analysis was used to identify the binding sites of CDX2 and HNF4α.
Figure 1. The researchers transfected Caco-2, T84, LS174T, DLD-1, SW480, and HT-29 cells with pGL4.10 luciferase reporter constructs containing the YAP1 promoter and +82 kb enhancer, measuring relative luciferase/β-galactosidase activity. HEK293 cells served as non-intestinal controls. (Larsen S, et al., 2019)
A: The expression level of luciferase reporter genes is usually higher in T84 cells to ensure the reliability and detectability of experimental signals. Specific expression levels can be quantified through luciferase activity assays. High expression levels mean that a clear luciferase signal can be obtained even with lower concentrations of inducers or inhibitors in experiments, thereby improving the sensitivity and accuracy of experiments.
A: Luciferase Reporter Cell Line - T84 is suitable for a variety of experimental designs, including drug screening, gene function research, signaling pathway analysis, etc. As a human colon cancer cell line, T84 cells are particularly suitable for studying signaling pathways and drug responses related to intestinal epithelial cells. For example, specific regulatory elements can be transfected, the regulatory effect of candidate drugs on these elements can be evaluated, and quantitative analysis can be performed through changes in luciferase activity.
A: The culture conditions of the T84 cell line need to be strictly controlled, including using appropriate culture media (such as RPMI-1640 containing 10% fetal calf serum) and maintaining a constant temperature culture environment of 37°C and 5% CO2. This cell line has been genetically edited to stably integrate the luciferase gene, thus maintaining stable luciferase expression during passage. This stability ensures the repeatability and reliability of experimental results, which is particularly important for long-term experiments.
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I have recently used the Luciferase Reporter Cell Line - T84 for gene expression monitoring and it feels very good. First of all, the merchant shipped the goods in a timely manner, and the cells were in good condition after receiving them. Although cells will form high-density aggregation during the culture process, good growth status can be maintained as long as the culture medium is replaced in time. Especially in monitoring transcriptional regulation and protein-protein interactions, the Luciferase signal is very clear and the data is reliable.
The Luciferase Reporter Cell Line - T84 used this time was very much in line with my expectations. Cell lines are particularly good at monitoring protein interactions and intracellular signaling, making them a great addition to the laboratory.
The experience using Luciferase Reporter Cell Line - T84 has been very good. The cells were packaged and shipped very professionally and were in excellent condition when received. The performance of the Luciferase reporter gene is also very stable and can accurately reflect changes in gene expression and signaling pathways. This cell line is very effective in studying cancer signaling pathways and is highly recommended.
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