Luciferase Reporter Cell Line - NALM6

Luciferase reporter cell line NALM6 is engineered to express luciferase as a reporter gene, allowing for the monitoring of gene expression levels in real-time. In 1976, Minowada et al. isolated NALM-6 cells from the blood culture of a male ALL patient who was 19 years old. A model for B-cell differentiation, invasion of the central nervous system (CNS), metastasis, and precursor B-cell leukemia is NALM-6, a cell line derived from B-cell leukemia. Reporter gene experiments can be performed with NALM-6 cells that have been modified to carry the reporter gene, making them perfect for evaluating the effectiveness of new CAR-T cell treatments or other immunotherapies in vivo. The appearance of NALM-6 cells is similar to that of lymphoblasts, with tiny, spherical, suspended cells. A near-diploid human karyotype is shown by chromosomal analysis. In immunocompromised mice, these cells grow into tumors and can spread spontaneously to the liver, spleen, lymph nodes, and bone marrow. The validity of NALM-6 cells is verified by STR profiling, and there is no evidence of mycoplasma contamination. For NALM-6 cells, RPMI-1640 media supplemented with 10% fetal bovine serum and 37°C culture temperatures with 5% carbon dioxide are the suggested culture conditions. To maintain cell density between 1×10^5 and 2×10^6 cells/ml, it is recommended to change the culture media two to three times a week. The cells have a doubling duration of roughly 36 hours.