Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR0554
Host Cell : MM1.R Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR0554 |
| Description | This cell line is engineered to stably express luciferase reporter gene in MM1.R. |
| Target Gene | Luciferase |
| Host Cell | MM1.R |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
MM.1R is a B lymphoblastoid cell line that was isolated in 1990 from the peripheral blood of a 42-year-old black woman with multiple myeloma who had developed resistance to steroid therapy. Multiple myeloma is a cancer caused by plasma cells, a type of white blood cell produced in the bone marrow. In people with multiple myeloma, malignant plasma cells accumulate in the bone marrow, crowding out normal plasma cells that help fight infection. MM.1R cells can be used in immune system disease research and immunology.
The luciferase reporter cell line - MM1.R is a novel and powerful tool for studying gene regulation and signal transduction, especially in live cell imaging. This cell line has been engineered to express luciferase, a light-emitting enzyme, under the control of specific regulatory elements or promoters. Introduction of a luciferase reporter gene into these cells allows real-time monitoring of gene expression and cell signaling pathways. When a specific promoter controlling the luciferase gene is activated, the enzyme is produced and emits light in the presence of its substrate luciferin. This emitted light can then be detected and quantified using a photometer or suitable imaging system, providing a direct measurement of the cellular event under study.
The Luciferase Reporter Cell Line - MM1.R is a valuable tool used in a variety of scientific and medical research fields. Here are some applications of this cell line:
Drug Discovery and Screening: The luciferase reporter cell line - MM1.R is widely used in the discovery and development of new drugs. These cell lines can be engineered to express specific target genes. The effect of the drug candidate can then be determined by measuring the gene's expression.
Gene Function Studies: The MM1.R reporter cell line can help researchers understand the function of a specific gene. By replacing this gene with a luciferase gene, researchers can observe what the gene does within the cell.
Signaling Research: Researchers frequently use the MM1.R reporter cell line to study cell signaling pathways. By introducing reporter genes into these cell lines, scientists can track specific signaling proteins and understand their interactions.
Cancer Research: The luciferase reporter cell line - MM1.R is used in cancer research to understand cancer progression. Researchers can introduce mutations and study their effects on cell growth or drug resistance.
Vaccine Development: The luciferase reporter cell line - MM1.R can be used for vaccine development and testing. By introducing disease-causing genes into these cell lines and then exposing them to vaccine candidates, researchers can measure the vaccine's effectiveness by looking at the gene's expression levels.
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We have been using the MM1.R luciferase reporter cell line for drug screening assays and it has performed very well. The cell line is easy to culture and maintain, and the luciferase signal is stable and reproducible.
The affordability and reliability of the MM1.R Luciferase Reporter Cell Line make it an excellent choice for academic and commercial labs alike.
One of the standout features of the MM1.R Luciferase Reporter Cell Line is its robustness under varying experimental conditions. Whether we are conducting long-term studies or short-term assays, the cell line consistently delivers high-quality, reproducible data.
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